Protein Sorting/ Targeting

Question 1

Protein destinations and if after or during translation (3)

Answer 1

Stay in cytosol
Mitochondria + nucleus - Post translational
Secretory pathway - Co-translational trafficking

Question 2

What determines sorting of protein in secretory pathway

Answer 2

Signal sequences

Question 3

What directions are proteins made

Answer 3

N-terminus to C-terminus (5’ to 3’ direction)

Question 4

Overview steps of post-translation (4)

Answer 4

stay in cytosol
Protein synthesis on free ribosomes
proteins folded
Transported to nucleus, mitochondria, chloroplast, peroxisomes

Question 5

In post translation: how is the protein sorted to the nucleus

Answer 5

Uses Nuclear