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protein structure Flashcards

protein analysis: explain the principles of electrophoresis, and why proteins with single mutations may be separated by electrophoresis depending on the individual mutations; explain the effects that post-translational modifications may have on protein analysis (3 cards)

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1
Q

how are proteins or amino acids analysed

A

electrophoresis

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2
Q

what allow mutated amino acids to be detected

A

lower RMM or glycosylation

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3
Q

what effect would a mutation creating cysteine (e.g. osteogenesis imperfecta) on electrophoresis

A

cysteine has a greater RMM so travels slower

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