Proteins-Primary

Question 1

Proteins expressed at high levels

Answer 1

Collagen, hemoglobin

Question 2

Proteins expressed at low levels

Answer 2

Hormones, signaling proteins

Question 3

Purification and analysis by charge

Answer 3

Ion exchange chromatography,
Isoelectric focusing,
Electrophoresis

Question 4

Protein purification and analysis by Size

Answer 4

Dialysis and ultracentrifugation,
Gel electrophoresis,
Gel filtration(size exclusion)
SDS-page

Question 5

By Charge and Size

Answer 5

2D gel electrophoresis

Question 6

Protein purification and Analysis by Specificity

Answer 6

Affinity Chromatography

Question 7

Protein Purification and Analysis by Polarity

Answer 7

Paper chromatography,
Reverse-phase chromatography,
Hydrophobic chromatography

Question 8

Liquid Chromatography

Answer 8

Uses buffer or solvent

Question 9

Edman uses

Answer 9

Enzymes

Question 10

Mass spec advantages

Answer 10

Specific
Sensitive
Identify several peptides from a protein
Multiple proteins at a time in parallel
Type of modification and exact location