Random for MT 1

Question 1

Microbiological growth inhibition assay - which tests?

Answer 1

a. Four-cup (plate) method
b. KIS (Kidney Inhibition Swab) test
c. Brillian-black reduction test
d. Delvo test

Question 2

Receptor-binding tests - which tests?

Answer 2

a. ROSA (Rapid One Step Assay) test
b. Beta star
c. Snap test
d. Meatsafe B-lactam

Question 3

Immune-assays - which tests?

Answer 3

a. Charm II test

b. ELISA

Question 4

Screening Methods

Answer 4

1. Microbiological growth inhibition assay
2. Receptor-binding tests
3. Immune-assays
4. Biosensor tests

Question 5

D

Answer 5

Decimal duction time - is the time needed for the live cell count of the population to decrease by one order of magnitude

Question 6

TDT

Answer 6

Thermal Death Time - The time necessary for ‘n’ decimal (order of magnitude) reductions in the live cell count of the population

Question 7

Z value

Answer 7

The temperature increase necessary for a decimal (tenfold) reduction for the thermal death time

Question 8

F value

Answer 8

Thermal death time at 121.1 C temperature - Microbiological characteristic which expresses the thermal death time at 121.1 C

Question 9

F0

Answer 9

Technological characteristic that indicates the effect of the actual heat treatment on Clostridium botulinum spores is equivalent with F0 minutes of heat treatment at 121.1 C

Question 10

12D principle

Answer 10

Food microbiology safety requirement that when growth and toxin production of Clostridium botulinum is possible in product (pH higher than 4.5), heat treatment applied should cause at least 12 log reduction in the number of spores.

Question 11

Heat treatment:

• N
• N0
• (delta)N/(delta)t
• k
• D
• TDT
• F0

Answer 11

• N: number of living cells
• N0: number of initial living cells
• (delta)N/(delta)t: destruction rate
• k: death rate coefficient or specific destruction rate
• D: decimal reduction time (for e.g. 10^7-10^6 = 1D)
• TDT - Thermal Death Time: The time necessary for n decimal reductions of the population (TDT=n x D)
• F: 12D – Thermal Death Time at 121.1 degrees
• F0: necessary time for the destruction of Clostridium botulinum by isothermal heat treatment at 121.1 degrees (2.52 minutes at 121.1 C)

Question 12

Monitoring programmes

Answer 12

• Monitoring of residues (foods of animal origin)
• Monitoring of chemical safety (foods of animal and plant origin)
• Monitoring of pesticides (food and products of plant origin)