RDNA Technology

Question 1

What are the steps for RDNA Technology?

Answer 1

Isolation of desired gene by using restriction enzymes which cuts DNA at specific sites.

Next, using the same restriction enzyme ,the plasmid linearized by being cleaved, creating a site for the insertion of the desired gene. Thus, obtaining a recombinant plasmid

Thereafter, the plasmid is introduced to a bacteria cell, through a process called transformation.

Finally, the bacteria cell divides and produces the desired genes

Question 2

Tools needed for RDNA Technology?

Answer 2

• Plasmids
• Restriction enzymes - Enzymes that cut DNA
• Gel electrophoresis
• Polymerase Chain Reaction (PCR)

Question 3

Processes needed in RDNA Technology?

Answer 3

• Linearization of plasmid (cutting of plasmid using the same restriction enzyme)
• Ligation
• Transformation (Introduction of recombinant plasmid to a host organism)

Question 4

Function of restriction enzyme

Answer 4

• To cut DNA at specific sites

Question 5

What is a vector?

Answer 5

It is a plasmid that is used to transfer DNA into a living cell

Question 5

What is a plasmid?

Answer 5

A small circular DNA used to transfer DNA into a living cell