1
Q
Part of the virus in which the envelope is required.
A
Nuclear/Cytoplasmic membrane
2
Q
Eosinophilic cytoplasmic inclusions are elementary bodies of ___virus.
A
Poxvirus
3
Q
Incubation period for fungi that grow on blood.
A
28 days
4
Q
Parasites that have neither a cyst form. (2)
A
Dientamoeba fragilis
Trichomonas vaginalis
5
Q
Roundworm that inhabits small intestine and usually demonstrated as rhabditoform larva.
A
Strongyloides stercoralis
6
Q
Calcium alginate swab is used for spx taken fr. nasopharynx for the isolation of ______.
A
Corynebacterium diphtheriae
7
Q
Used to isolate S.aureus from stool cultures.
A
Medium with 7.5%salt.
8
Q
These are used as counterstain for acid fast staining.
A
Malachite green
Methylene blue
9
Q
2 examples of anaerobic bacteria.
A
Veilonella
Actinomyces
10
Q
+ result for bile solubility test
A
Lysis of the semisolid media
11
Q
This bacteria does not contain a capsule.
A
N.meningitidis
12
Q
Bacteria that produces Babes-Ernst granules.
A
C.diptheriae
13
Q
How to prepare an agar medium?
A
Half water ➡️ Agar ➡️ Half water
14
Q
Composition of IODOPHORE
A
Iodine + Detergent
15
Q
Composition of TINCTURE OF IODINE
A
Iodine + alcohol
16
Q
Type of plasma used for Tube Coagulase test
A
RABBIT OR HUMAN PLASMA
17
Q
Lecithinase (+)
BULL’S EYE HEMOLYSIS
A
C.perfringens
18
Q
Lowest concentration of drugs that inhibits bacterial growth.
A
MIC
Min. Inhibitory Concentration
19
Q
Lowest concentration of drugs that kills/destroys organisms.
A
MLC
Min. Lethal Concentration
20
Q
Gram neg, COFFEE-BEAN shaped diplococci with adjacent sides flattened
A
NEISSERIA spp.
21
Q
Appropriate spx’s for culturing N.gonorrhea: (5)
A
(GREVO) Gastric fluid Rectum Eyes Vaginal discharge Oral Cavity
22
Q
Grows on CYSTEINE TELLURITE AGAR
A
C.diptheriae
23
Q
CA of WHOOPING COUGH
A
Bordatella pertussis
24
Q
It inhibits growth of gram pos and fungi in MAC agar.
A
BILE Salt
25
C.albicans mouth infection
THRUSH
26
Parasite that possess rhabditiform larva in stool.
S.stercoralis
27
Types of Plasmodium which accounts for 90% of cases (2)
P.vivax
| P.falciparum
28
Example of an ECTOPARASITE
CRABS 🦀
29
Most predominant form of Plasmodium causing MALARIA IN THE WORLD
Vivax
30
Most predominant form of Plasmodium causing MALARIA IN THE PHILIPPINES
Falciparum
31
Agent of GAY BOWEL SYNDROME
Giardia lamblia
32
3rd Taenia spp
Asiatica
*common name: Taiwan Taenia
33
Taenia spp having a uterus that is DICHOTOMOUS / TREE-LIKE BRANCHES 🌲
T.saginata
34
Taenia spp having a uterus that is DENDRITIC OR FINGER-LIKE 🤚
T.solium
35
The adult resembles the adult of SPIROMETRA
D.latum
36
Resembles the egg of P.westermani
D.latum
37
+ ctrl for ACETATE TEST
E.coli
38
Acetate test:
Incubation period
+ result
35’C for 7 days
| BLUE
39
ACETAMIDE TEST
+ctrl
INCUBATION PERIOD
+result
Pseudomonas aeruginosa
35’C for 7 days
BLUE
40
```
MALONATE TEST
+ CRTL
INCUBATION PERIOD
+ result
- result
```
Salmonella
35’C for 7 days
BLUE +
GREEN/YELLOW -
41
Used in KATO-THICK Smear preparation
Cellophane paper soaked in mixture of GLYCERINE + MALACHITE GREEN/METHYLENE BLUE SOL’N
42
For detection of stipplings, how long will you need to prepare blood films after the blood is drawn?
30 MINS to 1Hr
43
Site of the preferred blood spx for blood smear for PROTOZOA
Finger puncture
44
Definitive host to Plasmodium
Female anopheles mosquito
45
Color of CHROMATID BODIES WITH TRICHROME STAIN
BRIGHT RED
46
Appearance of glycogen in the trophozoite after staining with trichrome stain.
Nucleus?
COLORLESS : glycogen
RED-PURPLE : Nucleus
47
Appearance of Charcot Leyden crystals in the trophozoite after staining with trichrome stain.
BRIGHT RED
48
Chromatoidal bodies of protozoan cysts in iodine
DO NOT STAIN
49
Stains used for: NAEGLERIA and ACANTHAMOEBA
H&E
| WRIGHT’s stain
50
Destroyed by iodine
Trophozoites
51
Hexacanth embryo:
(Hymenolepis spp)
▪️polar filaments (+)
▪️polar filaments (-) lacks
H.nana (+)
| H.diminuta (-)
52
The egg of ___ have hexacanth embryo in a radially striated shell
Taenia
53
2nd IH of D.latum
FRESH WATER CRABS
| CRAYFISHES
54
Method for recovery of eggs of S.japonicum and S.mansoni
Rectal biopsy
55
These are HERMAPHRODITIC
Flukes
| Tapeworms
56
Nematode parasites capable of HEART LUNG MIGRATION
ASH
A.lumbricoides
S.stercoralis
Hookworms
57
“Unholy three” or “Triad of Infection”
HAT
Hookworms
A.lumbricoides
T.trichura
58
Ag that is cross-reactive in ALL ADENOVIRUSES
Hexon
59
CA of Farmer’s Lungs
Aspergillus
60
Media used to ID C.albicans through the organisms’ CHLAMYDOSPORES production
CORNMEAL AGAR PLUS TWEEN 80
61
C.albicans produces (3)
BLASTOSPORES
CHLAMYDOSPORES
PSEUDOHYPHAE
62
Stain in AMAN STAIN
| The most common fungal stain
LPCB
63
Setting of RPMs marked on the face of the RHEOSTAT CTRL ON THE CENTRIFUGE. Checking?
Monthly
64
Checking of:
CATALASE
OXIDASE
COAGULASE
Once each day of use
| When vial is 1st used
65
THIOGLYCOLLATE broth is boiled for ___ mins to?
10 mins
| Drive off O2
66
Clear medium for MYCOBACTERIA
Middlebrook 7H11
67
Bordatella spp with is both UREASE AND OXIDASE +
B.bronchiseptica
68
Preferred medium for the isolation of B.pertussis
CHARCOAL-CEPHALEXIN AGAR
69
Inhibitors in SALMONELLA-SHIGELLA AGAR (2)
Bile salts
| Brilliant green
70
Methods for testing Beta lactamase (3)
Chromogenic-Cephalosporin Mtd
Acidometric mtd
Iodometric mtd
71
Transcribe PPNG
Penicillinase-Producing Gonococci
72
ZOI:
SENSITIVE FOR OPTOCHIN DISK TEST (Taxo P)
For Bacitracin Disk Test
(Taxo A)
>|= 14mm (taxo P)
Any ZOI = Sensitive
73
Most common pathogen in throat cultures.
GROUP A Strep (S.pyogenes)
74
Staph that is DNAse:
+
-
S.aureus
| S.epidermidis
75
Carrier used in COAGGLUTINATION by staph.
Staphylococcal PROTEIN A
76
Can be used for Serotyping by swelling
Capsule
77
Milk is considered as GRADE A if the bacterial ct is _____ per mL when RAW and not exceed ____ bacteria per mL once pasteurized
75K
| 15K
78
Stain used to demonstrate spores
Schaeffer-Fulton
79
Nasopharyngeal swab spx is recommended for detection of (3)
B.pertussis
N.meningitidis
H.influenzae
80
Virus that can cause SHINGLES
VZV
81
Culture media for Fungi (3)
SBA
POTATO DEXTROSE AGAR
CORNMEAL AGAR
82
Broad spectrum antibiotics
Act against...
1. gram pos
2. gram neg
3. Bacterial and non bacterial
83
Number of nuclei of mature cyst of Entamoeba polecki
1
84
Heat resistant fungi commonly associated as FOOD CONTAMINANT
Aspergillus
85
Parasite that has characteristic AUTO-FLUORESCENCE
Cyclospora cayetanensis
86
Organism having grayish white, transparent to translucent, matte or glossy colonies with a large ZONE OF BETA HEMOLYSIS
Group A (S.pyogenes)
87
Types of blood for the detection of microfilaria are (2)
CAPILLARY
| ARTERIAL
88
Proglottid filled with eggs
Gravid
89
Virus that can affect CNS
RABIES
90
Most common mtd for toxoplasma detection
Detect specific ab
91
Continent where Chagas Dse was 1st discovered
America
92
Transcribe ToRCHeS
```
Toxoplasma gondii
Rubella
CMV
HERPES
HEPATITIS
SYPHILIS
```
93
This stains CELL WALL of fungi
Calcoflour white
94
Obligate microaerophiles
Campylobacter
| Helicobacter
95
Coccidia that can be contacted from cats
Toxoplasma gondii
96
Mechanical/phoretic vector or carrier of Ascaris eggs
Cockroaches
97
Toxic to viruses
Toxic to Neisseria spp
Toxic to Chamydia spp
Calcium alginate swab - ✖️viruses
Cottons swab - ✖️ Neisseria
Wood - ✖️Chlamydia
98
Chain of infection
Source
MOT
Susceptible Host
99
Appropriate ratio of blood:broth for BLOOD CULTURE
1:5
100
MOT OF HTLV I and II
Blood
Serum
Saliva
Semen
S3B
101
Best types of water to prepare an agar medium are:
Deionized
| Distilled
102
Is rejected for the the dx of RSV
Stool
103
Test used to determine C.tetani
Morphology
| Tennis racket; lollipop; DRUMSTICK
104
AFB FOUND IN NASAL MUCOSA
Not dx’c - NF
105
Dx’C lab media (agar) for bacterial isolation has pH of
7.0 to 7.5
106
Test used to diff. S pyogenes and S.pneumoniae from other strep.
Hemolysis test
Beta - S.pyogenes
Alpha - S.pneumoniae
107
A tests used to diff.
Listeria
Corynebacteria
BILE ESCULIN HYDROLYSIS TEST
MOTILITY TEST
LISTERIA +
CORYNEBACTERIA -
108
Filamentous bacteria which is the common source of making antibiotics
Streptomyces
109
Too much moisture in disk diffusion in growth in AST leads to:
Smaller ZOI
110
In SSA GIVE APPEARANCE OF:
Salmonella
Shigella
Salmonella:
▪️colorless colonies with Black centers ; H2S +
Shigella
▪️colorless colonies without Black centers ; H2S -
111
Best culture media for Salmonella culture: (3)
SSA
XLD
TETRATHIONATE
112
Sterilization in cans can kill:
endospores of:
▪️C.botulinum
▪️E.coli
And toxins
113
Concentration of H2O2 as DISINFECTANT
3%
114
Most ideal for GS/CS of ambulatory px
Random urine spx
115
Most ideal for GS/CS for BEDRIDDEN PX
Catheterized urine
116
Most ideal for GS/CS of WOMEN
Midstream clean-catch
117
Alternative disinfectant if Na hypochlorite (chlorox) is unavailable in the lab.
Vinegar (acetic acid)
118
Refers to the property to see the cellular details that are microscopically micrometers
Resolution
119
Can be ruled out in SBA Plate
Haemophilus (fastidious org: HACEK)
120
```
Bacitracin - R
Hippurate hydrolysis test - neg
Bile esculin hydrolysis - pos
6.5% NaCl - pos
Beta hemolytic
```
Enterococcus
121
2 purpose of SIM (Sulfide Indole Motility)medium
▪️motility
| ▪️tryptophan medium
122
Soap is:
Germicidal
123
Microsporum that fluoresce on WOOD’s UV LAMP
M.canis
124
Agar for DERMATOPHYTES
Mycosel Agar
125
This qualifies sputum for culture
GS
126
Test that speciates Neisseria spp.
Sugar Utilization (CHO UTILIZATION TEST)
127
Bacteria causes UTI (3)
E.coli
S.saprophyticus
Enterococci
128
Complicate CYSTIC FIBROSIS PX (2)
P.aeruginosa
| Burkholderia cepacia
129
Causes SWIMMER’S Ear and burns
P.aeruginosa
130
Acceptable spx for S.aureus
Nasal swab
131
Niacin +
Nitrate reduction +
Catalase -
MTB
132
Lecithinase +
| Double zone of hemolysis
C.perfringens
133
Four bacteria that are INHIBITED BY SPS DISK TEST:
G.vaginalis
Neisseria
Streptobacillus moniliformis
Peptostreptococcus anaerobius
134
Phenylalanine Deaminase (PAD) +
Urease+
Lysine decarboxylase +
PPM
PROTEUS
PROVIDENCIA
MORGANELLA
135
Test that has a positive result of RED: (5)
```
INDOLE
MR
VP
UREASE
NITRATE
```
136
Test that has a positive result of GREEN, neg color of BLUE: (4)
CAMA
Citrate
Acetamide
Malonate
Acetate utilization tests
137
Test that has a positive result of PURPLE: (4)
L2OH
LIA
LOA (decarboxylase)
Oxidase
hippurate hydolysis
138
Test that has a positive result of YELLOS: (3)
TOT
TSI
ONPG
TCBS (thiosulfate-citrate-bile salt-sucrose)
139
Salmonella spp that is LYSINE & H2S neg
S.paratyphi A
140
Test that diff Enterobacteriaciae
IMViC
141
Doesn’t grow on MAC: (3)
MEK
Moraxella
Eikenella corrodens
Kingella kingae
142
Pigment producer and causes grape-like odor on CETRIMIDE AGAR
P.aeruginosa
143
Pigment produced by P.aeruginosa
Pyocyanin
Pyoverdin
Pyorubin
144
Grows in CAP,
✖️MAC
✖️BAP
Haemophilus
145
Bacteria known for its requirement cysteine which causes lab acquired infxn : BSL III
Francisella tularensis
146
BSL I
My BaS
Mycobacterium gordonae
Bacillus subtilis
147
BSL II
Y Ba?
Yersinia pestis
B.anthracis
148
BSLIII
My FRiend BruMo
Mycobacteria
Francisella
Brucella
Molds
149
Can speciate Brucella spp.
Thionine
| Fuchsin
150
Brucella spp
CO2 +
H2S +
Inhibited by THIONINE
B.abortus
151
“Safety pin” appearance in Wayson’s stain (bipolar bodies)
| ▪️ acquired through animal bite wounds
Pasteurella
152
```
Causes:
▪️MENINGITIS
▪️SEPSIS
▪️STILLBIRTH
▪️FOOD POISONING
```
Listeria
153
The ONLY GRAM POS BACILLUS
H2S +
Catalase -
“BUTCHER’s CUT”
Erysipelothrix rhusiopathiae
154
CA of PONTIAC FEVER (fr. Aircondition)
| CM: BCYE
Legionella
MICROBIOLOGY (CUA)
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