Recombinant

Question 1

Key characterists of a plasmid

Answer 1

-easy to work with
-self replicating
-stable
-functional
1000-25000 base pairs

Question 2

The Process

Answer 2

1. DNA and plasmid are cut with same restricting enzymes
2. two sticky binds stick together
3. replicate
4. remove the damaged ones
5. add ampicillin and

Question 3

Transgenic animals

Answer 3

foreign things introduced to animal
desried proteins are expressed in milk
cheap

Question 4

transgenic plants

Answer 4

human albumin can be expressed in potatoes

-stability is an issue

Question 5

how to remove an agent from a system

Answer 5

1. filtration
2. preciptation
3. chromatography

Question 6

formulation of bio products considerations?

Answer 6

1. microbiological - sterility, pyrogens
2. exciptiatants - solubility enhancers, , anti-aggregations, anti-oxidants, buffers ect
3. self life - freeze dry proteins
4. route