Required practical 6

Question 1

What is evolution?

Answer 1

Evolution is the change in allele frequency over many generations in a population.

Question 2

What is aseptic technique?

Answer 2

Used to prevent contamination therefore u can get reliable and repeatable results.

Question 3

How is aseptic technique achieved (5 points)?

Answer 3

• Work in the convection current of a bunsen burner (carries bacteria in the air away).
• Wipe surfaces with disinfectant before and after.
• Minimise opening of containers with bacteria.
• Flame neck of any bottles used (before&after use).
• Flame inoculating loop used.

Question 4

Why only lightly tape the lid on the agar plate?

Answer 4

So oxygen can enter the plate and prevent the growth of pathogenic bacteria which prefer anaerobic conditions.

Question 5

Why incubate at 25 degrees celsius?

Answer 5

To prevent pathogenic bacteria from growing.

Question 6

What is the inhibition zone?

Answer 6

The area where bacteria have not grown (antibiotic inhibits the growth of that bacteria).