Review

Question 1

what kind of a virus is HIV?

Answer 1

a retrovirus with an RNA genome

Question 2

what is the role of a denaturing agent in gel electrophoresis?

Answer 2

disrupts hydrogen bonds and other interactions (i.e. natural structure is disturbed) causing it to unfold into a linear chain

Question 3

what is the role of a reducing agent in gel electrophoresis?

Answer 3

disrupts disulfide bonds

Question 4

what is the role of native state gel electrophoresis?

Answer 4

proteins retain their conformations

Question 5

what is k cat

Answer 5

number of substrate molecules each enzyme converts to product per unit time (turnover number)

Question 6

how do you find the efficiency of an enzyme?

Answer 6

k cat/Km

Question 7

k 2 is also known as

Answer 7

k cat

Question 8

V max =

Answer 8

k 2*[E total]