Rh group (Molbio-Rh function)

Question 1

Condition in which an antibody from the mother’s serum crosses the placental barrier and attacks the fetus’ cell;

Answer 1

HDN (Hemolytic disease of the Newborn)

Question 2

What chromosome are RHD and RHCE
found?

Answer 2

Chromosome 1

Question 3

present on most D-positive and all C- positive RBCs

Answer 3

Rh antigens – G antigen

Question 4

variants or mutations of Rh antigen differs about how many amino acids

Answer 4

32-35 amino acids

Question 5

RHD protein is flanked by

Answer 5

Rhesus boxes

Question 6

at what residue does Serine was replaced by Asparagine

Answer 6

residue 68

Question 7

Number of exons for RHD and RHCE

Answer 7

10

Question 8

What is the most immunogenic antigen and the least immunogenic?

Answer 8

D > c > E > C > e
D: is the most immunogenic
e: is the least immunogenic

Question 9

Isoleucine at residue 60 replaced

Answer 9

Leucine

Question 10

Commericially produced and purified IgG class Anti-D w/ a latex molecule attached

Answer 10

Rh Immune Globulin (RhIG)

Question 11

C and c differ by ____ amino acids

Answer 11

four

Question 12

sources of Rh antigen-G antigen (3 pts)

Answer 12

RhD, RhCe, and RhCE protein

Question 13

Where is RHAG located

Answer 13

Chromosome 6

Question 14

links Rh complex to the membrane skeleton

Answer 14

Rh/RhAG-ankyrin interaction and CD47-protein 4.2 association

Question 15

Serine at residue 68 replaced by

Answer 15

Asparagine

Question 16

Rh Function

Answer 16

(1)Structural integrity of Red Cells
(2) Ammonia and CO2 transporters

Question 17

She proposed that Rh was based on two Rh gene

Answer 17

Patricia Tippet

Question 18

RHAG appears early in what progenitor

Answer 18

CD34+

Question 19

E and e differ in what amino acid

Answer 19

Proline at residue 226 replaced by Alanine

Question 20

replaced by tryptophan at residue 16

Answer 20

Cysteine

Question 21

RHCE gene codes for

Answer 21

RhCe, RhcE, Rhce, RhCE

Question 22

True or False: RHAG cannot express Rh antigen alone

Answer 22

True

Question 23

RHD gene codes for

Answer 23

RHD protein

Question 24

Biochemically speaking, what type of molecules are Rh antigens?

Answer 24

Proteins

Question 25

Maintains structural integrity for the RBC

Answer 25

Rh antigens – “D”

Question 26

Located on chromosome 6, its product is similar in structure to the Rh proteins, with the difference being it is glycosylated. - also called as COEXPRESSOR

Answer 26

Rh- associated glycoprotein (RhAG)

Question 27

Rh antigen "D" amino acid

Answer 27

416

Question 28

G-antigen is originally found on rr person who received:

Answer 28

D+C- E-c+e+

Question 29

RH and RHAG mRNAs are restricted to what cells

Answer 29

Erythroid lineages

Question 30

E and e differ by ____ amino acid

Answer 30

one

Question 31

four major allelic forms of RHCE:

Answer 31

ce, Ce, cE, and CE

Question 32

encodes E and e on the 4th extracellular loop

Answer 32

exon 5

Question 33

Anti-LW will not react with which of the following? a. Rh-positive RBCs b. Rh-negative RBCs c. Rhnull RBCs d. Rh:33 RBCs

Answer 33

Rhnull RBCs

Question 34

Rh Positive contains these gene

Answer 34

RHD AND RHCE

Question 35

number of times Rh antigen "D" traverses the cell membrane

Answer 35

12 times

Question 36

Rh antibodies are primarily of which immunoglobulin class?

Answer 36

IgG

Question 37

Single point mutation in Rhce resulted in

Answer 37

RHcE

Question 38

important in determining C or c

Answer 38

Position 103

Question 39

Single gene produces a single product that contains separately recognizable factors

Answer 39

Wiener

Question 40

differentiates E from e

Answer 40

Position 226 –

Question 41

A gene important for Rh antigen expression

Answer 41

RHAG

Question 42

ability of a particular substance(antigenorepitope) to provoke an immune responsetothebody of a human or animal

Answer 42

Immunogenecity

Question 43

Results from the amino acid serine at position 103 on the RhD, RhCe, and RhCE protein

Answer 43

Rh antigens – G antigen

Question 44

serine to proline happens in

Answer 44

residue 103

Question 45

Rh gene chromosome

Answer 45

Chromosome 1

Question 46

Percentage identity of RHAG gene to Rh indicidual

Answer 46

47%

Question 47

Rh locus contains 3 distinct genes that control production of their respective antigen

Answer 47

Fisher race

Question 48

Serine at residue 103 replaced by

Answer 48

Proline

Question 49

replaced by Proline at residue 103

Answer 49

Serine

Question 50

Where does Rh antigwn-"D" resides

Answer 50

Transmembranous proteins