RP2

Question 1

Where in plants can cells undergoing mitosis be found?

Answer 1

Meristem tissue at shoot and root tips

Question 2

What is the mitotic index?

Answer 2

The ratio of cells undergoing mitosis to the total number of cells in a sample

Question 3

Outline the procedure to prepare a root tip slide

Answer 3

1. Warm 1M HCI to 60°C in a water bath.
2. Cut a root tip using a scalpel and add to the HCI. Leave for 5 minutes.
3. Remove from HI and wash with distilled water.
4. Cut the tip of the root tip sample and place on a slide.
5. Add a few drops of stain to make chromosomes visible

Question 4

What is the formula for the mitotic index

Answer 4

Mitotic index = Number of cells with visible chromosomes/ number of cells in sample