RPR

Question 1

What is a non-treponemal test for serologic detection of the antibody reagin, which is produced in response to an infection (syphilis) caused by Treponema pallidum?

Answer 1

Rapid Plasma Reagin

Question 2

What is produced in response to an infection (syphilis) caused by Treponema pallidum?

Answer 2

Antibody Reagin (phospholipid)

Question 3

So what is RPR testing for?

Answer 3

Syphilis

Question 4

Where is RPR found?

Answer 4

Serum or plasma of patients with syphilis

Question 5

What is used as a vehicle/antigen to see results macroscopically?

Answer 5

Carbon Charcoal

Question 6

Does RPR required confirmation?

Answer 6

Yes (therefore it is a screening test)

Question 7

Fluorescent treponemal antibody-absorbed test (FTA-ABS)

Answer 7

Dead T. pallidum is fixed on a slide, and patient’s serum is added

Question 8

FTA ABS (FAT ABS) is what?

Answer 8

Confirmatory test for RPR

Question 9

FAT ABS detects antibody how?

Answer 9

• By addition of fluorescein-labeled anti-human globulin.

* under ultraviolet microscope, it will fluoresce if positive

Question 10

What bacteria is associated with RPR?

Answer 10

Treponema pallidum

Question 11

What lipid is the antibody regain?

Answer 11

Phospholipid

Question 12

Non-reactive test results on rpr show?

Answer 12

No flocculation

Question 13

What is the monospot test used for?

Answer 13

detect infectious mononucleosis which is a self limiting disease caused by the Epstein-Barr virus

Question 14

When is mono typically seen?

Answer 14

young adults and children less than 5 years of age

Question 15

How does mono present?

Answer 15

fever, malaise, lethargy, sore throat with exudates, enlarged lymph nodes on the neck, mild hepatitis, enlarged spleen, and occasional blotchy skin rash

Question 16

What accumulation does mono cause?

Answer 16

increased numbers and abnormal forms of lymphocytes and monocytes in the lymph nodes

Question 17

What antibody is formed due to mono?

Answer 17

Heterophil

Question 18

What is a heterophil antibody?

Answer 18

Copy of the present antibody

Question 19

How is serum sample taken for rpr?

Answer 19

• Collect venous blood in tubes without anticoagulant (red top and sst)
• After centrifugation, serum should be clear and unhemolyzed

Question 20

What test is performed for RPR?

Answer 20

RPR card test kit

Question 21

Prior to using test card kit what must you do for rpr?

Answer 21

• Centrifuge the specimen (serum or plasma) to sediment cellular elements
• Mix the antigen suspension, prior to opening ampule, by shaking vigorously for 10-15 seconds
• Attach the needle to the hub of the dispensing bottle. Ensure the antigen is below the break line
• Snap off the top of the ampule and draw all of the antigen into the dispensing bottle

Question 22

How do you mix the antigen suspension in the card kit for rpr?

Answer 22

• Shake VIGOROUSLY for 10-15 seconds

Question 23

What must be used as a confirmatory test for RPR card kit test?

Answer 23

FAT ABS (FTA-ABS)

Question 24

What is the speed and time the card kit spins on the rotator for rpr?

Answer 24

8 minutes at 100 revolutions per minute

Question 25

How often do you rotate the card to differentiate the non-reactive from the weakly reactive results?

Answer 25

3 or 4 to and from

Question 26

What are the results on the test kit for rpr?

Answer 26

Reactive or Non-reactive

Question 27

How do you know it’s reactive?

Answer 27

Flocculation is present

Question 28

When are Rapid Plasma Reagin controls conducted?

Answer 28

always be run with each batch of Rapid Plasma Reagin tests

Question 29

How does quality control start?

Answer 29

with proper collection and handling of specimen

Question 30

How is quality control divided?

Answer 30

* Equipment quality control | * Reagent quality control

Question 31

What is the mechanical rotor calibrated to?

Answer 31

100 rpm

Question 32

What is the antigen needle calibrated to?

Answer 32

60 drops per 1 ml

Question 33

What controls are used for rpr?

Answer 33

* reactive * weak-reactive * non-reactive

Question 34

How are controls treated?

Answer 34

As patients and run every time an unknwon sample is tested