RR16

Question 1

Explain two types of genetic analysis

Answer 1

• Forward genetic analysis : “what gene is responsible for mutant?” - disrupt homeostasis based on random mutation. Start with phenotype and go to gene
• Reverse genetic analysis: “what is consequence of mutant on the organism?” - disrupt activity of specific gene product to assess it’s function. start with mutant and go to phenotype

Question 2

What are transgenes?

Answer 2

Aritfically integrated gene
- position it in different positions to see how it impacts gene expression
- Important in understanding gene expression patterns

Question 3

Explain CRISPPR/Cas9

Answer 3

• Cas 9 protein cuts DNA, Guide RNA recognizes DNA sequence to be edited
• Scientists create specific guide RNA for ‘problem’ and attach it to Cas9

Complex is introduced to target cell - cut DNA at target sequence
Now have the target sequence to be edited by scientists