Normal rumen pH
6.3-7
Slightly acidic
Normal VFA concentration in rumen.
80-120 mmol/L
Normal bacterial concentration of ruminate fluid.
10^7 - 10^12 per mL ruminate fluid
Normal ammonia concentration in ruminate fluid.
6-20 mmol/L
Low
Normal chloride concentration in the ruminal fluid.
15-20 mmol/L
Normal nitrate reduction test
No nitrite after 10 min because microorganisms have reduced it.
Normal methylene blue reduction test
Colourless (with blue ring) after 3-6 minutes.
Normal triphenyltetraxolium chloride (TTC) test
Red colour after 10-15 minutes.
Normal VFA composition of rumen.
60-65% acetate
20-25% propionate
10-15% butyrate
5% other acids