SAXS

Question 1

Which other protein structure technique is SAXS similar to?

Answer 1

X-ray crystallography

Question 2

Does SAXS have a good resolution?

Answer 2

No, we only get a shape

Question 3

Where is the protein when using SAXS?

Answer 3

In solution

Question 4

If we can only get a shape from using SAXS, why do we use it at all?

Answer 4

Can be very powerful when used along with x-ray crystallography or NMR. Pretty useless on its own

Question 5

What are the pros of SAXS?

Answer 5

No crystal, protein is in solution, get information on protein flexibility

Question 6

What are the cons of SAXS?

Answer 6

Very low resolution, shape only