Science Microscopes Flashcards

(36 cards)

1
Q

Who was the first person to make microscopes?

A

Antonie van Leeuwenhoek

He was the first person to see single-celled organisms and called them ‘animalcules’.

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2
Q

Who was the first scientist to use the word ‘cell’?

A

Robert Hooke

He used the word ‘cell’ because the cork cells he observed reminded him of the small rooms monks lived in.

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3
Q

What are the two important parts of the cell theory developed by Schleiden and Schwann?

A
  • All living things are composed of cells
  • The cell is the basic unit of life
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4
Q

What is the best magnification that you can get with a light microscope?

A

2000x

To see smaller parts of cells, an electron microscope is used.

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5
Q

How would the edge of a razor blade appear under a light microscope?

A

Blurry and not very detailed

Under an electron microscope, it would appear much clearer and show detailed structures.

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6
Q

What is a microscope?

A

An instrument used to see objects that are too small for the naked eye

Microscopes can be optical, electron, or other types depending on their function.

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7
Q

What is the eyepiece?

A

The part you look through to see the specimen; usually has 10x magnification.

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8
Q

Where is the eyepiece located?

A

At the very top of the microscope, above the tube.

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9
Q

What is the tube?

A

The long part that connects the eyepiece to the objective lenses.

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10
Q

Where is the tube located?

A

Between the eyepiece and the revolving nosepiece.

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11
Q

What is the coarse adjustment knob?

A

The large knob used to focus on the specimen roughly by moving the stage up or down.

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12
Q

Where is the coarse adjustment knob located?

A

On the side of the microscope, usually the bigger of the two focusing knobs.

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13
Q

What is the fine adjustment knob?

A

The small knob used to fine-tune the focus after the coarse adjustment.

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14
Q

Where is the fine adjustment knob located?

A

Next to or inside the coarse adjustment knob on the side.

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15
Q

What is the arm?

A

The part you hold to carry the microscope; supports the tube and stage.

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16
Q

Where is the arm located?

A

On the side, connecting the base to the tube and stage.

17
Q

What are the objective lenses?

A

The lenses closest to the specimen; provide different magnification powers.

18
Q

Where are the objective lenses located?

A

On the revolving nosepiece, just above the stage.

19
Q

What is the stage?

A

The flat platform where you place the slide.

20
Q

Where is the stage located?

A

In the middle of the microscope, under the objective lenses.

21
Q

What is the light source?

A

The part that shines light upward through the slide to make the specimen visible.

22
Q

Where is the light source located?

A

At the base of the microscope, under the stage.

23
Q

What does magnification mean in a microscope?

A

Magnification means how much larger an object looks compared to its real size.

Example: 100x means the image looks 100 times bigger.

24
Q

What does resolution mean in a microscope?

A

Resolution is how clear and detailed the image looks. Higher resolution means you can see smaller details.

25
How do you calculate total magnification on a microscope?
Multiply the eyepiece magnification by the objective lens magnification. ## Footnote Example: eyepiece 10x × objective 40x = 400x total.
26
If the eyepiece lens is 10x and the objective lens is 4x, what is the total magnification?
40x (10 × 4).
27
If the eyepiece lens is 10x and the objective lens is 40x, what is the total magnification?
400x (10 × 40).
28
If the eyepiece lens is 10x and the objective lens is 100x, what is the total magnification?
1000x (10 × 100).
29
Q: What is the first step in preparing a wet mount slide?
A: Place a clean glass slide on the work surface.
30
Q: After placing the slide, what should you do?
A: Put a drop of water in the centre of the slide using a dropper.
31
Q: What should you do after adding the drop of water?
A: Place the specimen (e.g., onion skin) into the drop.
32
Q: How do you position the coverslip before lowering it?
A: Hold the coverslip at a 45° angle to the slide.
33
Q: Why should you lower the coverslip slowly at an angle?
A: To reduce the chance of trapping air bubbles.
34
Q: Once the coverslip is in place, what should you do?
A: Gently press down with the flat side of a pencil or tweezers to remove extra water.
35
Q: What is the final step before putting the slide under the microscope?
A: Use a paper towel to blot away excess water around the edges.
36
Q: Why is it important to remove excess water around the slide?
A: To prevent the slide from slipping and to keep the microscope clean.