Phosphatase
Remove phosphate from a substrate
Kinase
moves phosphate from ATP to a substrate
Phosphorylase
moves phosphate from inorganic phosphate to a substrate
What is kcat?
kcat is used to describe the rate-limiting step of catalysis under saturating conditions of substrate.
uncompetitive inhibitor
presence of an uncompetitive inhibitor results in a lower apparent KM and Vmax of the enzymatically catalyzed reaction
anion exchange column binds
anions