For a refractometer to obtain accurate levels of protein, its concentrations
in the sample should be:
between 2- 2.5 g/dL
not more than 2.5 g/dL.
greater than 25 g/dL
greater than 2.5 g/dL
greater than 2.5 g/dL
A sulfosalicylic acid filtrate
When the Jaffe reaction is employed as a kinetic assay to quantitate serum
creatinine, which of the following is used in the analysis?
A sulfosalicylic acid filtrate
The serum sample is used directly
A Somogyi-Neson filtrate
A Folin-Wu filtrate
The serum sample is used directly
The creatinine clearance test is routinely used to assess the glomerular
filtration rate. Given the following information for an average size adult,
calculate the creatinine clearance. Urine creatinine-120 mg/dLPlasma
creatinine-1.2 mg/dLUrine volume for 24 hours-1520 mL *
94.7 mL/min
10.6 mL/min
105.6 mL/min
63.3 ml/min
105.6 mL/min
Among the polyanion-divalent cations for HDL-C measurement, this is the
only one that agrees closely with those obtained using analytical or
preparative ultracentrifugation: *
Dextran sulfate –Mg
Heparin-Ca
Sodium phosphotungstate-Mg
Heparin/ Mn++ precipitant
Heparin/ Mn++ precipitant
Also known as orosomucoid
prealbumin
Ceruloplasmin
α1-acid glycoprotein
Gc-globulin
α1-acid glycoprotein
Patient’s test for serum triglyceride this morning resulted to 220mg/dl.
Later in the afternoon, you remembered you didn’t remove the free
glycerol. How much should have been his result if only you did its
removal? *
80-90 mg/dl
200-210mg/dl
105-110 mg/dl
180-190 mg/dl
200-210mg/dl
A patient’s blood has been standing for 2 hours, since the previous Med
Tech on duty has left it beside the centrifuge by negligence. The next
technologist performed the ammonia test which gives a result of 1.336
µg/mL. How much was its initial content and its actual interpretation? *
1.3 µg/mL, increased
1.136 µg/mL, increased
13.0 µg/mL, decreased
1.3036 µg/mL, increased
13.0 µg/mL, decreased
Proteins absorb UV light due to the presence of the following EXCEPT: *
phenylalanine
tryptophan
threonine
tyrosine.
threonine
This involves the
development of a violet
color from the reaction
of ninhydrin (tri
ketohydrindine hydrate)
and primary amines.
This involves the
development of a violet
color from the reaction
of ninhydrin (tri
ketohydrindine hydrate)
and primary amines.
Coomassie
Brilliant
Blue Dye
In this reaction, the
ammonia liberated by
digestion is allowed to
react with alkaline
hypochlorite to form
indophenol blue solution.
The catalyst used is
sodium nitroprusside.
In this reaction, the
ammonia liberated by
digestion is allowed to
react with alkaline
hypochlorite to form
indophenol blue solution.
The catalyst used is
sodium nitroprusside.
Berthelot
reaction
This involves reaction of
the liberated ammonium
ions with double iodide
of potassium and
mercury to form
dimercuric ammonium
iodide which is a yellow
to orange brown product.
Nesslerization
reaction
This is used to detect
peptides and amino
acids after paper
chromatography.
This is used to detect
peptides and amino
acids after paper
chromatography.
Coomassie
Brilliant
Blue Dye
This method of protein
determination is based
on the ability of
phosphotungstomolybdic
acid to oxidize the
phenolic structures of
the amino acids tyrosine,
phenylalanine,
tryptophan and histidine.
This method of protein
determination is based
on the ability of
phosphotungstomolybdic
acid to oxidize the
phenolic structures of
the amino acids tyrosine,
phenylalanine,
tryptophan and histidine
Folin
Ciocalteau
Method
Which of the following Amino acids are negatively charged? *
Aspartic Acid
Glutamic Acid
Histidine
Lysine
First 2 choices only
Last 2 choices only
Any of the above
First 2 choices only
Most of the fat soluble vitamins are deficient in this condition due to
malabsorption: *
Abetalipoproteinemia
Hypobetalipoproteinemia
Chylomicron retention
Abetalipoproteinemia
In determining the Lipid Profile, which procedure / computation is NOT
acceptable: *
VLDL-cholesterol = total cholesterol – infranatant-cholesterol
LDL-cholesterol = infranatant cholesterol – HDL-cholesterol
Determine first TAG, and total cholesterol HDL-cholesterol by chemical means
Total-cholesterol = infranatant cholesterol - TAG
Total-cholesterol = infranatant cholesterol - TAG
Deficiency of this protein leads to Wilson’s disease (Hepatolenticular
Degeneration) *
Ceruloplasmin
α1-acid glycoprotein
Gc-globulin
prealbumin
Ceruloplasmin
The main endpoint of Alkoxide trans-esterification is the production of this
substance from the liberation of glycerol: *
lipoproteins
NADH
H2O2
Formaldehyde
Formaldehyde
Cystinuria
reabsorption
of positively
charged
amino acids
Glutaric
acidemia type 2
proteins
fats
metabolism
Alkaptonuria
tyrosine &
phenylalanine
Type II
citrullinemia
urea
cycle
The following statements are true regarding Uric acid determination
EXCEPT: *
Ethanol ingestion frequently increases the plasma concentration
It is readily oxidized to allantoin
It may be oxidized by alkaline
phosphotungstate
Using the enzyme catalase remains the most specific method
Using the enzyme catalase remains the most specific method
Homocystinuria
methionine