HISTOPATH > Staining & Mounting > Flashcards

Staining & Mounting Flashcards

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1
Q

Components are demostrated by DIRECT INTERACTION with Dye or staining solution

A

HISTOLOGICAL STAINING

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2
Q

Components are studied through chemical reaction; for demonstration of CHEMICAL COMPONENTS

A

Histochemical / Histochemistry staining

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3
Q

Histochemical staining used for HEMOGLOBIN

A

Pearl’s Prussian Blue reaction

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4
Q

Histochemical staining used for Carbohydrates

A

Periodic Acid Schiff

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5
Q

Demonstrate tissue ANTIGEN

A

Immunohistochemical staining

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6
Q

This is a combination of immunologic and histochemical staining

A

Imunnohistochemical staining

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7
Q

Detection of phenotypic markers that are detected by antibody

A

Immunohistochemical

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8
Q

Staining technique that uses aqueous or alcoholic dye to produce color

A

Direct Staining

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9
Q

Type of staining techniques that uses one dye and color of the dye is usually the resulting color

TISSUE + DYE = COLOR

A

Direct Staining

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10
Q

Type of staining that uses MORDANT or ACCENTUATOR to produce color

A

Indirect Staining

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11
Q

Substance that serves as the link/bridge between the dye and the tissue

A

MORDANT

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12
Q

EXAMPLES OF MORDANT

A

Erlich
Cole
Mayers
Gills
ALUM ( potassium alum)

Iron
- Heidenhain = ferric ammonium sulfate
- Weigerts = ferrim ammonium chloride

Copper = copper
PTAH = phophotungstic acid

ALL ARE HEMATOXYLINS

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13
Q

Substance that INCREASES you he color intensity and selectivity of the dye

A

ACCENTUATOR

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14
Q

EXAMPLES OF ACCENTUATOR

A

KOH
Phenol in carbol fuchsin and carbol thionine

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15
Q

Staining technique that use gradual application of dye. Tissue elements is re-stained in DEFINITE SEQUENCE

A

Progressive staining

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16
Q

Dye is applied little by little until desired color is achieved

A

Progressive staining

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17
Q

This staining technique do not need DECOLORIZATION

A

Progressive staining

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18
Q

Tissue is overstained and excess dye should be removed using DECOLORIZATION

A

Regressive staining

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19
Q

Selectively removal of excess of dye

A

DECOLONIZATION OR DIFFERENTIATION

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20
Q

This is the most common DECOLORIZATION substance and can remove both ACID and BASIC dye

A

ACID ALCOHOL

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21
Q

This of type of staining technique that involves application of different color to produce contrast and background

A

counterstaining

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22
Q

This of type of staining technique that involves application of different color to produce contrast and background

A

counterstaining

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23
Q

Type of staining technique that stained tissue with a color that is DIFFERENT from the stain itself

A

Metachromatic staining

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24
Q

Which stain is not belong to Metachromatic staining

Methyl violet
Cresyl blue
Safranin
Nile blue
Methylene blue

A

Nile blue

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25
This staining technique stained tissue with the SAME SHADE /HUE as that if the dye
Orthochromatic staining
26
Type of staining technique that is Selective to living cells
Vital staining
27
Two types of Vital staining
Intravital & Supravital
28
The dye/stain is so injected directly to any part of the LIVING BODY
Intravital
29
Stains is applied immediately to the tissue after its REMOVAL from living body
Supravital
30
Best vital stain
Neutral red
31
Especially recommended for MITOCHONDRIA under supravital staining
Janus green
32
type of staining technique that DO NOT USE DYE instead use METALLIC SALTS
Metallic IMPREGNATION
33
Leaves black deposits on the surface of the tissue
Metallic impregnation
34
Which of these metallic salt cannot be used? Ammoniacal silver Silver nitrate Ammoniacal gold Gold chloride
Ammoniacal gold (There is no ammoniacal gold)
35
Most common method that utilized MICROANATOMICAL STUDIES OF TISSUE
H&E Staining
36
Dye used in cytoplasmic stain; counterstaining
EOSIN
37
TRUE OR FALSE tissue fixed with mercuric chloride staining time should be DECREASED slightly while duration of eosin should be INCREASED
False staining time should be INCREASED duration should be REDUCED
38
H&E result color for Nuclei
Blue to blue black
39
H&E color result for Karyosome
Dark blue
40
H&E color result for Cytoplasm
Pale pink
41
H&E color result for RBC and Eosinophilic granules
Keratin-bright orange red
42
H&E color result for Calcium and decalcified bone
Purplish blue
43
H&E color result for decalcified bone MATRIX, collagen and osteoid
PINK
44
H&E color result for muscle fibers
Deep pink
45
what are the staining methods for FROZEN section
H&E Thionine Polychrome methylene blue Alcoholic pinacyanol method
46
Derived from plants and animals
Natural Dyes
47
Not a stain, active coloring agent is HEMATEIN
Hematoxylin
48
Mordant of hematoxylin
Alum, iron, copper, chromium
49
Hematoxylin mordant that is also a general for photomicrography
Iron hematoxylin
50
Under alum hematoxylin, ripening agents for exfoliative cytology and demonstration of sex chromosome
Harris hematoxylin
51
Under alum hematoxylin a ripening agent that use sodium iodate with citric acid & chloral hydrate as preservative for immunohistochemistry
Mayers hematoxylin
52
Mordant of Weigert’s hematoxylin for muscle fibers and CT
Ferric ammonium CHLORIDE
53
Mordant of Heidenhain’s hematoxylin for nuclei &cytoplasmic inclusion
Ferric ammonium SULFATE
54
Demonstrate spermatogenesis
Copper hematoxylin
55
Demonstrate endocrine cells of alimentary tract
Lead hematoxylin
56
Demonstrate of collagen, reticulin and argentaffin cells
6 Molybdenum hematoxylin
57
Extracted from bug (Coccus cacti)
Cochineal dye
58
For neutropatholigical studies
Pictic acid picro carmine
59
Natural dye for glycoge
Aluminum chloride best carmine
60
Vegetable oil extracted from lichens used for staining ELASTIC FIBERS
Orcein
61
Vegetable dye extracted from lichens but is NOT USED IN CYTOLOGICAL STAIN
Litmus
62
Aka COAL TAR DYES / ANILINE DYES
Synthetic dyes
63
Responsible for coloring property giving imparting color
Chromophore
64
Responsible for dyeing property retaining imparted color
Auxochrome
65
Without auxochrome component that demonstrates fats
Lysochrome
66
Most sensitive of oil soluble dyes
Sudan black B
HISTOPATH
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