Stains

Question 1

What are the 5 types of Stains?

Answer 1

1. Giemsa
2. PAS (Periodic acid-Schiff)
3. Ziehl-Neelsen (carbol fuchsin)
4. India ink
5. Silver stain

Question 2

How would you observe Treponemes?

Answer 2

1. Dark-field microscopy

2. Fluorescent antibody staining

Question 3

How would you observe Legionella?

Answer 3

Silver stain

Question 4

Bugs stained with Giemsa?

Answer 4

Certain Bugs Really Try my Patience
Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium

Question 5

Bugs stained with PAS (Periodic acid-Schiff)?

Answer 5

Tropheryma whipplei for diagnosing Whipple dz

Question 6

What does PAS stain?

Answer 6

Glycogen, mucopolysaccharides

Question 7

Bugs stained with Ziehl-Neelsen (carbol fuchsin)

Answer 7

Acid-fast organisms

1. Nocardia
2. Mycobacterium

Question 8

Bug stained with India ink

Answer 8

Cryptococcus neoformans

Question 9

To stain Cryptococcus neoformans, other than india ink, what stain can be used?

Answer 9

Mucicarmine can also be used to stain thick polysaccharide capsule red

Question 10

Bugs stained with silver stain

Answer 10

1. Fungi (Pneumocystis)
2. Legionella
3. Helicobacter pylori