Sterilisation

Question 1

what is asepsis?

Answer 1

exclusion of microorganisms and spores

Question 2

what is antisepsis?

Answer 2

prevention of sepsis by destruction or inhibition of microorganisms using an agent that may be safely applied to tissue

Question 3

what are some goals of sterilisation?

Answer 3

• to kill all forms of life
• reduces the risk of transmission of infectious agents

Question 4

what are some types of sterilisation?

Answer 4

• heat (dry, steam)
• cold (chemicals, ethylene oxide, gamma)

Question 5

what are some factors that affect rate of infection?

Answer 5

• time lapse between cleaning
• location and number of microorganisms
• temperature
• environmental pH
• organic matter
• type of organism
• insufficient cleaning

Question 6

what are brownes tubes?

Answer 6

liquid filled glass tubes that contain a heat sensitive dye that will change colour

Question 7

what is a spore test?

Answer 7

a strip is placed in a hot air oven and then cultured

Question 8

what temperature and time does glassware have to be sterilised for in a dry heat oven?

Answer 8

180 degrees c for 60 mins

Question 9

what temperature and time does powders and oils have to be sterilised for in a dry heat oven?

Answer 9

160 degrees c for 120 mins

Question 10

what temperature and time do sharp instruments have to be sterilised for in a dry heat oven?

Answer 10

150 degrees c for 180 mins

Question 11

what are four types of cold sterilisation?

Answer 11

• ethylene oxide
• gamma
• chemical
• alcohol

Question 12

what temperature does ethylene oxide steriliser operate at?

Answer 12

37-63 degrees

Question 13

what can be sterilised by gamma rays?

Answer 13

consumables

Question 14

what are some chemical sterilisation methods?

Answer 14

• QAC
• Alcohol
• bleach