Stras: Semen

Question 1

1. Maturation of spermatozoa takes place in the:
   A. Sertoli cells
   B. Seminiferous tubules
   C. Epididymis
   D. Seminal vesicles

Answer 1

C. Epididymis

Question 2

2. Enzymes for the coagulation and liquefaction of semen are produced by the:
   A. Seminal vesicles
   B. Bulbourethral glands
   C. Ductus deferens
   D. Prostate gland

Answer 2

D. Prostate gland

Question 3

3. The major component of seminal fluid is:
   A. Glucose
   B. Fructose
   C. Acid phosphatase
   D. Citric acid

Answer 3

B. Fructose

Question 4

4. If the first portion of a semen specimen is not collected, the semen analysis will have an abnormal:
   A. ph
   B. Viscosity
   C. Sperm concentration
   D. Sperm motility

Answer 4

C. Sperm concentration

Question 5

5. Failure of laboratory personnel to document the time a semen sample is collected primarily affects the interpretation of semen:
   A. Appearance
   B. Volume
   C. ph
   D. Viscosity

Answer 5

D. Viscosity

Question 6

6. Liquefaction of a semen specimen should take place within:
   A. 1 hour
   B. 2 hours
   C. 3 hours
   D. 4 hours

Answer 6

A. 1 hour

Question 7

7. A semen specimen delivered to the laboratory in a condom has a normal sperm count and markedly decreased sperm motility. This is indicative of:
   A. Decreased fructose
   B. Antispermicide in the condom
   C. Increased semen viscosity
   D. Increased semen alkalinity

Answer 7

B. Antispermicide in the condom

Question 8

8. An increased semen ph may be caused by:
   A. Prostatic infection
   B. Decreased prostatic secretions
   C. Decreased bulbourethral gland secretions
   D. All of the above

Answer 8

D. All of the above

Question 9

9. Proteolytic enzymes may be added to semen
   specimens to:
   A. Increase the viscosity
   B. Dilute the specimen
   C. Decrease the viscosity
   D. Neutralize the specimen

Answer 9

C. Decrease the viscosity

Question 10

10. The normal sperm concentration is:
    A. Below 20 million per microliter
    B. Above 20 million per milliliter
    C. Below 20 million per milliliter
    D. Above 20 million per microliter

Answer 10

B. Above 20 million per milliliter

Question 11

11. Given the following information, calculate the
    sperm
    concentration: dilution, 1:20; sperm counted in five
    RBC squares on each side of the hemocytometer, 80
    and 86; volume, 3 mL.
    A. 80 million per milliliter
    B. 83 million per milliliter
    C. 86 million per milliliter
    D. 169 million per microliter

Answer 11

B. 83 million per milliliter

Question 12

12. Using the above information, calculate the sperm concentration when 80 sperm are counted in 1 WBC square and 86 sperm are counted in another WBC square.
    A. 83 million per milliliter
    B. 166 million per ejaculate
    C. 16.6 million per milliliter
    D. 50 million per ejaculate

Answer 12

C. 16.6 million per milliliter

Question 13

13. The primary reason to dilute a semen specimen before performing a sperm concentration is to:
    A. Immobilize the sperm
    B. Facilitate the chamber count
    C. Decrease the viscosity
    D. Stain the sperm

Answer 13

A. Immobilize the sperm

Question 14

14. When performing a sperm concentration, 60 sperm are counted in the RBC squares on one side of the hemocytometer and 90 sperm are counted in the RBC squares on the other side. The specimen is diluted 1:20. The:
    A. Specimen should be rediluted and counted
    B. Sperm count is 75 million per milliliter
    C. Sperm count is greater than 5 million per milliliter
    D. Sperm concentation is abnormal

Answer 14

A. Specimen should be rediluted and counted

Question 15

15. Sperm motility evaluations are performed:
    A. Immediately after the specimen is collected
    B. Within 1–2 hours of collection
    C. After 3 hours of incubation
    D. At 6-hour intervals for one day

Answer 15

B. Within 1–2 hours of collection

Question 16

16. Sperm motility is evaluated on the basis of:
    A. Speed
    B. Direction
    C. Tail movement
    D. Both A and B

Answer 16

D. Both A and B

Question 17

17. The percentage of sperm showing average motility that is considered normal is:
    A. 25%
    B. 50%
    C. 60%
    D. 75%

Answer 17

B. 50%

Question 18

18. All of the following are grading criteria for sperm motility except:
    A. Rapid straight-line movement
    B. Rapid lateral movement
    C. No forward progression
    D. No movement

Answer 18

B. Rapid lateral movement

Question 19

19. The purpose of the acrosomal cap is:
    A. Ovum penetration
    B. Protection of the nucleus
    C. Energy for tail movement
    D. Protection of the neckpiece

Answer 19

A. Ovum penetration

Question 20

20. The sperm part containing a mitochondrial sheath is the:
    A. Head
    B. Neckpiece
    C. Midpiece
    D. Tail

Answer 20

C. Midpiece

Question 21

21. All of the following are associated with sperm motility except the:
    A. Head
    B. Neckpiece
    C. Midpiece
    D. Tail

Answer 21

A. Head

Question 22

22. The morphologic shape of a normal sperm head is:
    A. Round
    B. Tapered
    C. Oval
    D. Amorphous

Answer 22

C. Oval

Question 23

23. Normal sperm morphology when using the WHO criteria is:
    A.> 30% normal forms
    B. <30% normal forms
    C. >15% abnormal forms
    D. <15% normal forms

Answer 23

A.> 30% normal forms

Question 24

24. Additional parameters measured by Kruger strict morphology include all of the following except:
    A. Viability
    B. Presence of vacuoles
    C. Acrosome size
    D. Tail length

Answer 24

A. Viability

Question 25

25. Round cells that are of concern and may be included in sperm counts and morphology analysis are: A. Leukocytes B. Spermatids C. RBCs D. Both A and B

Answer 25

D. Both A and B

Question 26

26. If 5 round cells per 100 sperm are counted in a sperm morphology smear and the sperm concentation is 30 million, the concentraton of round cells is: A. 150,000 B. 1.5 million C. 300,000 D. 15 million

Answer 26

B. 1.5 million

Question 27

27. Following an abnormal sperm motility test with a normal sperm count, what additional test might be ordered? A. Fructose level B. Zinc level C. Mar test D. Eosin-nigrosin stain

Answer 27

D. Eosin-nigrosin stain

Question 28

28. Follow-up testing for a low sperm concentration would include testing for: A. Antisperm antibodies B. Seminal fluid fructose C. Sperm viability D. Prostatic acid phosphatase

Answer 28

B. Seminal fluid fructose

Question 29

29. The immunobead test for antisperm antibodies: A. Detects the presence of male antibodies B. Determines the presence of IgG, IgM, and IgA antibodies C. Determines the location of antisperm antibodies D. All of the above

Answer 29

D. All of the above

Question 30

30. Measurement of _x0007_-glucosidase is performed to detect a disorder of the: A. Seminiferous tubules B. Epididymis C. Prostate gland D. Bulbourethral gland

Answer 30

B. Epididymis

Question 31

31. A specimen delivered to the laboratory with a request for prostatic acid phosphatase and glycoprotein p30 was collected to determine: A. Prostatic infection B. Presence of antisperm antibodies C. A possible rape D. Successful vasectomy

Answer 31

C. A possible rape

Question 32

32. Following a negative postvasectomy wet preparation, the specimen should be: A. Centrifuged and reexamined B. Stained and reexamined C. Reported as no sperm seen D. Both A and B

Answer 32

A. Centrifuged and reexamined

Question 33

33. Standardization of procedures and reference values for semen analysis is primarily provided by the: A. Manufacturers of instrumentation B. WHO C. Manufacturers of control samples D. Clinical laboratory improvement amendments

Answer 33

B. WHO