MTAP1.3 - MYCOVIRO - MIDTERMS > Temporary Mounts > Flashcards

Temporary Mounts Flashcards

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1
Q

Dissolves non-fungal material, digest debris, to make fungal elements
more visible (acts as a clearing agent)

A

10-20% KOH Concentration

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2
Q

Skin, hair

A

10% KOH

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3
Q

Nails (hard tissues)

A

20% KOH

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4
Q

Most widely used staining method for fungi

Acts as both preservative and stain

A

Lactophenol Cotton Blue

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5
Q

Lactic Acid

A

Preservative

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6
Q

Phenol

A

Killing agent

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7
Q

Cotton Blue

A

Stain

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8
Q

Negative stain renders the organism colorless and colors the background

A

India Ink / Nigrosin

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9
Q

India Ink / Nigrosin demonstrates capsule of

A

Cryptococcus neoformans

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10
Q

Fluorochrome dye – best for direct microscopy

A

Calcofluor White Stain

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MTAP1.3 - MYCOVIRO - MIDTERMS
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