tissue engineering

Question 1

what are the advantages and disadv of CaOH pulp capping?

Answer 1

1. excellent antibacterial properties
   2) induces min of dentin
2. low cytotoxicity

disadv:

1. high soluble in oral fluids
2. subject to dissolution
3. presence of funnels in reparative dentin
4. lack of adhesion

Question 2

what are the adv and disadv of ZOE as a pulp cap?

Answer 2

adv:
1. reduces inflammation

disadv:

1. lack of calcfic bridge formation
2. release eugenol inhigh conc–>cytotoxicity
3. interfacial leakage

Question 3

what are the adv and disadv of corticosteroids and abx for pulp capping?

Answer 3

adv:
1. reduces pulp inflammation
2. vancomycin and CaOH stimulate more reparitive dentin

disadv:
1. should not be used in pts with risk from bacterimia

Question 4

what are the adv and disadv of polycarboxylate cements as pulp capping material?

Answer 4

adv:
1. chem. bonds to tooth

disadv:

1. lacks antibacterial effects
2. does not stimulate calcific bridge formation

Question 5

what are the adv and disadv of inert materials like ISOBUTYL CYANOACRYLATE and CaPO3

Answer 5

adv:
1. reduces pulp inflammation
2. stims dentin bridge formation

disadv:
1. not viable bc of lack of technique

Question 6

what are the adv and disadv of collagen as a pulp cap mateiral?

Answer 6

adv:
1. less irritating, promotes mineralizatiopn

disadv:
1. does not help in thick dentin bridge formation

Question 7

describe calcium phosphate as a pulp cap material

Answer 7

adv:

1. helps in bridge formation with no superficial necrosis
2. no inflammation
3. good physical properties

disadv:
1. must perform clinical trials to evaluate this mateiral

Question 8

what are the adv and disadv of hydroxyapatite as a pulp capmaterial?

Answer 8

adv:

1. biocompatible
2. scaffold for new dentin formation

disadv:
1. mild inflammation with superficial necrosis of pulp

Question 9

what are the adv and disadv of CO2 lasers

Answer 9

adv

1. formation of secondary dentin
2. bacterialcidal effects

disadv

1. technique sensitive
2. cause thermal damage to pulp at high doses

Question 10

what are the adv and disadv of GI/RMGI as a pulp cap material?

Answer 10

adv

1. excellent bacterial seal
2. fluoride release,
3. CTE and modulus of elasticity similar to dentin
4. good biocompatibility

disadv

1. causes chronic inflammation
2. lack of dentin bridge formation
3. cytotoxic when in direct cell contact
4. high solubility and slow setting rate

Question 11

what are the adv and disadv of MTAas a pulp capping material

Answer 11

adv

1. good biocompatiblity
2. not much pulpal inflammation
3. more predictable hard tissue barrier
4. radiopacity

disadv

1. expensive
2. poor handling characteristics
3. two step procedure
4. high solubility

Question 12

what are the adv and disadv of growth factor as a pulp capping material?

Answer 12

adv

1. formation of osteodentin and tubular dentin
2. formation of more homogenous reparative dentin
3. superior to CaOH for mineralization

disadv

1. high conc required
2. half life is less
3. appropriate dose response is required to prevent obliteration of pulp chamber

Question 13

what are the adv and disadv of odontogenic meloblast associated proteins

Answer 13

adv

1. biocomaptible
2. accelerates reactionary dentin formation
3. does not promote excessive teriary dentin formation

disadv:
1. only in vitro studies conducted
\2. further studies required

Question 14

what are the adv and disadv of thera cal as a pulp capping material?

Answer 14

adv

1. protect pulp
2. strong physical properties
3. no solubility
4. radiopaque
5. good Ca release

disadv
1. can affect composite shading–unesthettic

Question 15

what ar ethe factors that determine the success ofpulp capping?

Answer 15

• remaining dentin thickness

- choice of indirect pulp capping agent

Question 16

what is the purpose of cyanoacrylate

Answer 16

• placed over wound site
• polymerizes in the presence of moisture
• forms a PROTECTIVE COATING

Question 17

what are the adv and disadv of cyanoacrylate monomer?

Answer 17

adv

• good biocompatilbity (only butyl and isobutyl forms)
• good tissue adherence
• biodegradable–>wound heals and it breaks down
• provides hemostasis
• bacteriostatic
• easy to apply (to small surfaces)
• shortens operation time

disadv:

• difficult to apply to large wounds
• is toxic in methyl form

Question 18

what is n-butyl-2-cyanoacrylate and what is it used for?

Answer 18

a tissue adhesives, not cytotoxic –> can be used instread of stitches and parrier from food/debris during the healing period

Question 19

what are the absorbable sutures?

Answer 19

PGA
PDO
catgut

Question 20

what are the non-absorbable sutures?

Answer 20

silk
polyester
nylon

Question 21

what is an important benefit of an alloplast?

Answer 21

do not pose the risk of transmitting disease

Question 22

what is guided tissue regeneration?

Answer 22

undesired cells excluded from repopulating a defects or injury site by placing a physical barrier; desriable cells can enter

Question 23

describe cell induction as tissue regeneration?

Answer 23

growth and differentiation factors are injected; can also create gene vectors and cause growth/diff factors to be produced endogenously

Question 24

describe cells as a scaffolding matrix as a strategy for tissue engineering

Answer 24

preformed scaffolds are seeded with cells from a pt; grown in vitro –> increase in # of cells–> start producing matrix; with enough growth, implanted back into pt, cells grow and develop into tissues; scaffold resorbs and leaves no former prescence

Question 25

strategries for tx engineering?

Answer 25

1. injection of cells -- right at site of injury 2. guided tissue regeneration 3. cell induction -- 4. cells in a scaffold matrix

Question 26

what does SIBLINGs stand for

Answer 26

small integrin bindin ligand, N-linked glycoprotein - includes bone sialoprotein (BSP), - osteopontin (OPN) - dentin sialophosphoprotein (DSPP) - dentin-matrix protein 1 (DMP-1) - matrix extrecellular pohosphoprotein (MEPE) - noncollagenous - mediate cell adhesion

Question 27

what does allotropic mean?

Answer 27

that an element can exist in different physical forms

Question 28

what materials are used in perio, and for what purpose?

Answer 28

used to promote healing - ZOE - Non-eugenol (zinc polyacrylate, resin) cyanoacrylate molecule