Tissue Processing

Question 1

What are the 3 main characteristics of tissue samples used for observation with a microscope?

Answer 1

1. well-preserved during fixation using formalin
2. sufficiently thin using a microtome (1-7 μm)
3. contrasted using staining

Question 2

What are the 5 major steps of tissue processing?

Answer 2

1. fixation (formalin)
2. dehydration (ascending % of alcohol)
3. clearing (xylene)
4. infiltration (fluid)
5. embedding (solid paraffin wax)

Question 3

What is the microtome used for?

Answer 3

sectioning paraffin-embedded tissue into 1-7 μm (usually 5) thick segments

Question 4

What process is done to the tissue after it goes through the microtome?

Answer 4

staining