Xylem
Phloem Vessels
Sclerenchyma
sclerenchyma fibres are also found within vascular bundles and provide structural support to the plant. They are made up of bundles of long, dead cells. The cells have a hollow lumen and the cell walls are thickened with lignin.
Plant Fibres
Plant fibres, such as cotton and hemp, are made up of long tubes of plant cells. Plant fibres are strong which makes them useful for materials such as rope or sacks. Their strength is due to two factors:
The arrangement of cellulose microfibrils in the cell wall
The secondary thickening of cell walls with lignin
CPAC - Dissecting Plant Stems
Cut a thin section of the plant stem using a scalpel. Take care when using the sample and remember to cut away from you.
Place the tissue sample into water to prevent it from drying out.
Place the tissue sample into a small dish containing the stain. A common stain that is used to view vascular bundles is toluidine blue O (TBO) which stains lignin blue/green which will enable you to visualise the xylem and sclerenchyma fibres. The phloem cells and remaining tissue will appear a pink/purple colour.
Rinse the tissue samples in water and place each one onto a microscope slide.