1
Q
Method
A
Quantitative Enzymatic Colorimetric Method (Stanbio)
2
Q
Principle
A
Cholesterol esterase hydrolyzes cholesterol esters into free cholesterol and fatty
acids
All free cholesterol in the sample is oxidized by cholesterol oxidase into cholest
4-ene-3-one and H2O2
A chromogen is produced when phenol is oxidatively coupled with 4
aminophenazone in the presence of peroxidase with H2O2
3
Q
Wavelentgh
A
500 nm
4
Q
End color
A
Pink
5
Q
Conversion factor
A
0.026
6
Q
Conventional unit
A
150 - 250 mg/dL
7
Q
SI unit
A
3.88–6.47 umol/L
CC 1 MSPE
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