Transcriptomics/Genomics

Question 1

Put in the correct order the steps of sample preparation and
RNA-Sequencing:

a) PCR
b) Sequencing
c) Abundant transcript removal
d) RNA purification
e) Fragmentation
f) cDNA Synthesis
g) Adapter ligation

Answer 1

1. d) RNA Purification
2. c) Abundant transcript removal
3. e) Fragmentation
4. f) cDNA Synthesis
5. g) Adapter Ligation
6. a) PCR
7. b) Sequencing

Question 2

STAR stores the genome index as (Single choice):
a) Hash
b) Suffix Array
c) De Bruijn graph
d) BWT

Answer 2

b) Suffix Array