Where does the Rbp4 line express Cre?
L5 cortical neurons
What is the purpose of Rbp4?
Control deep-layer excitatory neurons
Where does the Ntsr1 line express Cre?
L6 corticothalamic neurons
What is the purpose of Ntsr1?
Target corticothalamic circuits
What does the Cux2 line express?
Tamoxifen-inducible Cre
Where does the Cux2 line express Cre?
L2/3-4 neurons
What is the purpose of Cux2?
Time-controlled labelling
What does the H2B line express?
Nuclear GFP
Where is the H2B line expressed?
Cre dependent, reporter line
What is the purpose of the H2B line?
permanent nuclear labelling
What does the ArchT line express?
ArchT is an optogenetic silencer and a light-actiavted proton pump used to inhibit neuronal activity.
Activated by green/yellow light
Pumps H+ ions out of cell
Causes membrane hyperpolarisation
=Neuronal silencing/inhibition
Usually fluoresecently tagged for visualization
Where is the ArchT line expressed?
Cre dependent
What is the purpose of the ArchT line?
Optical silencing of neurons
