Describe the flow of info in the Central Dogma
DNA - mRNA - protein
What is formed by deamination of adenosine
inosine
Wobble base pairing
some tRNAs recognize more than one codon
The formation of a peptide bond b/w free aa is thermodynamically unfavourable so what activated intermediates do we need for protein syntheis?
amino acyl tRNA
What are the two steps in tRNA charging
- the aminoacyl-AMP is transferred to tRNA
2. Adenylation and transfer is catlayzed by amino acyl tRNA sythtase
Polycistronic
a single strand that can be encoded for many different proteins
What is the shine dalgerno seq
Upstream of the start codon ribosmal bidnign site
What are some post-translational protein modifications
aa side chains that are most commonly modifed have hydroxy, amino, or thiol functional groups
tRNA
adaptor molecules b/w nucleic acid and protein
How are aa activated and linked to a specific tRNA
by aminoacyl-tRNA sythetase
ribsome
a large complex of protein and RNA
Proteins are modified after translation
Key functional groups are commonly covalently modified to alter cheisty and protein function
