Translation

Question 1

tRNA loading

Answer 1

• load with AA by tRNA synthetase

- attach covalently

Question 2

ribosome

Answer 2

• 30S controls mRNA decoding site

- 50S contains peptidyl transferase that helps form peptide bonds

Question 3

IF1

Answer 3

induces ribosome to dissociate

Question 4

IF3

Answer 4

binds to 30S submit forming IF3-30S-mRNA complex to prevent reassociation of ribosome

Question 5

IF2

Answer 5

• complexes with GTP and initiator tRNA-fmet

- directs binding of tRNA to 30S

Question 6

IF3 dissociates

Answer 6

• 50S subunit reassociates
• GTP hydrolyzes - GDP, IF3, and Pi leave
• fmet-tRNA on top of start codon in P site

Question 7

elongation

Answer 7

A site accepts next tRNA

Question 8

Ef-Tu

Answer 8

• brings next tRNA with use of GTP and moves to A site

- forms peptide bond

Question 9

23 subunit

Answer 9

• helps form peptide bond

Question 10

Ef-G

Answer 10

• helps transfer tRNA from A site to P site with help of GTP

- tRNA goes to E site and leaves

Question 11

Termination

Answer 11

• termination codon enters A site

- Ef-Tu can’t bind - translation stalls

Question 12

RF1/RF2

Answer 12

release polypeptide from tRNA in P site

Question 13

RF3

Answer 13

removes RF1/RF2