Define transpiration
This is the process of water loss in form of water vapour to the atmosphere from the plant.
What are the types of transpiration?
i. Stomatal transpiration
ii. Cuticular transpiration
iii. Lenticular transpiration
Describe the three types of transpiration
Stomatal
- This is the loss of water vapour to the atmosphere through the stomatal pores of the leaves.
- This contributes 90% of the total water loss from a leafy shoot.
- This is because leaves contain a large number of stomata for gaseous exchange where this water vapour can pass and also there’s little resistance to the movement of water vapour through the stomatal pores.
- In addition, leaves also have a large surface area over which water vapour can evaporate rapidly to the atmosphere.
Cuticular transpiration
- This is the loss of water vapour to the atmosphere directly through the epidermis coated with a cuticle layer.
- It contributes 5% to the total water loss from the leafy shoot.
- This is because the cuticle is hard, waxy and less permeable to most diffusing molecules including water vapour molecules.
Lenticular transpiration
- This is the loss of water vapour through a mass of loosely packed cells known as lenticels found scattered on the stems.
- It also contributes 5% of the total water loss to the atmosphere in a leafy shoot.
- It is because the lenticels are usually few in number and not directly exposed to environmental conditions.
- Lenticular transpiration is the main source of water loss from deciduous plants after shading off their leaves. Because there are more stomata on the leaves than elsewhere in the shoot system, it is evidence that most of the water vapour is lost from the leaves.
Describe the structure of a lenticel
What are hydathodes?
Modified stomata found in flowers and leaf margins capable of guttation
What is guttation?
The loss of water from a plant in liquid form.
Describe the structure of a stoma
What assumption is made when using weighing method to determine rate of transpiration?
What assumption is made when using potometer method to determine rate of transpiration?
What precautions are taken when using a potometer?
What is the ecological significance of transpiration?
What is a criticism of the photosynthetic product theory of stomatal opening and closing?
The theory does not explain how the low rate of glucose formation can account for the rapid opening of stomata
What are the advantages of transpiration?
What are the disadvantages of transpiration?
What are the effects of wilting?
What is the ecological significance of transpiration?
What is the physiological significance of transpiration?
What factors affect transpiration?
Environmental:
- Temperature
- Humidity
- Wind/ air currents
- Light intensity
Non-environmental:
- Number of stomata
- Leaf area
- Cuticle
- Leaf arrangement
(Check book for explanations)
What conditions favor stomatal opening?
Describe the photosynthetic product theory of stomatal openings and closing
Describe the active potassium theory of stomatal opening and closing in plants
In light:
- Starch is converted to malic acid in guard cells
- Malic acid dissociates into malate ions and hydrogen ions
- Blue light activates ATPase and hydrogen ions are actively pumped out of guard cells into neighboring epidermal cells as potassium ions are actively pumped into the guard cells.
- Accumulation of malate ions and potassium ions in the guard cells lowers the water potential of the guard cells.
- However in some plants such as onions where the guard cells have no starch, malate does not accumulate and instead chloride ions are taken up with positive ions to maintain the electrochemical neutrality.
- Water enters into the guard cells by osmosis and guard cells become turgid, stomata open
In darkness:
- Potassium ions diffusion out of the guard cells into neighboring epidermal cells
- Malic acid converts back into starch
- Hydrogen ions re-enter the guard cells via carrier proteins, lowering the pH of guard cells
- Water potential of the guard cell sap increases above that of neighboring epidermal cells
- Water moves out of guard cells into neighboring epidermal cells, they become flaccid and the stomata close
Describe the starch-sugar interconversion theory of stomatal opening and closing
In the day:
- Guard cells photosynthesize
- Decreased concentration of CO2 in guard cells
- Increased pH (alkaline)
- Starch is converted to sugar
- The cell sap concentration increases thus the water potential decreases
- Water enters the guard cells by endosmosis
- Increased turgor in the guard cell
- Stomata open
At night:
- Photosynthesis in guard cells ceases
- Increased concentration of CO2 in guard cells
- Decreased pH (acidic)
- Sugar is converted to starch
- The cell sap concentration decreases thus the water potential increases
- Water leaves the guard cells by exosmosis
- Decreased turgor in the guard cell
- Stomata close
What is the sequence of layers in the root?
Epidermis > Cortex > Endodermis (which has suberin) > xylem vessel
Describe the mechanism of water uptake by plants (ie into the endodermal cells)
Water flows from root hairs endodermal cells using three pathways, namely;
a) Apoplast (cell wall) pathway
b) Symplast (cytoplasm) pathway
c) Vacuolar pathway
Apoplast pathway
- This is the pathway in which water moves through the spaces between the cellulose fibres in the cell wall of one cell to the cell wall of the adjacent cells.
Symplast pathway
- This is the movement of water through the cytoplasm of one cell to the cytoplasm of the adjacent cell via plasmodesmata.
Vacuolar pathway
- This is the movement of water from the sap vacuole of one cell to the sap vacuole of the adjacent cell following a water potential gradient.