Unit 1.1f

Question 1

What is the point in aseptic techniques?

Answer 1

Prevent contamination by microorganisms of cell cultures and also prevent accidental inoculation

Question 2

What are microbe’s cultured on?

Answer 2

Broth (suspended liquid medium) or on /within agar (solid medium)

Question 3

What do different media’s promote?

Answer 3

The growth of different cell types and microbes

Question 4

What are optimum growth conditions provided in?

Answer 4

Nutrients, pH, gasses

Question 5

What is step 1 in inoculation?

Answer 5

Wash hands with antibacterial soap or alcohol to reduce bacteria

Question 6

What is step 2 in inoculation?

Answer 6

Swap area you are working in with disinfectant to sterilise the area

Question 7

What is step 3 in inoculation?

Answer 7

Using permanent pen label base of agar plate:
your name, microbes name, date, class

Question 8

What is step 4 in inoculation?

Answer 8

Flame metal inoculating hoop until it glows red

Question 9

What is step 5 in inoculation?

Answer 9

Remove lid off the culture vessel using pinky finger, gently flame lip of culture vessel

Question 10

What is step 6 in inoculation?

Answer 10

In the Bunsens air canopy, pick up the sterile hoop and remove some of the yeast culture

Question 11

What is step 7 in inoculation?

Answer 11

Keep hold of loop, reframe lip and replace lid

Question 12

What is step 8 in inoculation?

Answer 12

Working in the Bunsen’s canopy, pick up base of sterile agar plate and with inoculating loop gently streak a pattern to dilute your culture of yeast microbes

Question 13

What is step 9 in inoculation?

Answer 13

Place face of inoculated agar plate onto its lid, secure lid onto base using two small strips of cello-tape

Question 14

What is step 10 in inoculation?

Answer 14

Place inoculate plate, lid down, into the incubator

Question 15

When growing in a culture what must the cells get a chance to have?

Answer 15

A gas exchange

Question 16

What does a typical culture include?

Answer 16

Water, salt, amino acids, vitamins, glucose

Question 17

What is essential in an animal cell culture medium?

Answer 17

A serum with growth promoting proteins

Question 18

What is plating out?

Answer 18

It’s when a liquid microbial culture is placed on a solid culture to allow the number of colony forming units to be counted and cell density to be estimated

Question 19

What is needed to achieve colony count?

Answer 19

Serial dilution

Question 20

What is a total cell count?

Answer 20

A count of all cells dead and alive

Question 21

What is a viable cell count?

Answer 21

The number of alive cells that are actively growing/dividing

Question 22

What is used to estimate the total viable cell count?

Answer 22

A haemocytometer

Question 23

What is used so cells can be seen when using a haemocytometer?

Answer 23

A vital stain

Question 24

What is a vital stain?

Answer 24

One that only stains either living or dead cells

Question 25

Why don’t dies work on most living cell?

Answer 25

The pump them out

Question 26

What is a common die used in yeast and mammalian cell cultures?

Answer 26

Trypan blue dye (stains dead cells only)

Question 27

What is a haemocytometer?

Answer 27

A specialised slide that has a counting chamber and a known liquid volume