UNIT 2 ACID FAST STAINING

Question 1

Kinyoun mordant

Answer 1

TERGITOL

Question 2

is used as the primary stain

Answer 2

Carbol fuchsin

Question 3

kinyoun acid alcohol NAF

Answer 3

colorless

Question 4

ACID FAST STAINING Principle:

Answer 4

Mycolic acid, once stained by the primary stain, is able to resist the decolorization of acid-alcohol rendering them as “ACID FAST”

Question 5

ZIEHL-NEELSEN ACID-FAST STAINING PROCEDURE

Answer 5

1. Cover smear with
   carbolfuchsin. Steam over
   boiling water for 8 minutes.
   Add additional stain if the
   stain boils off.
2. After the slide has cooled,
   decolorize with acid-alcohol
   for 15-20 seconds.
3. Stop decolorization action of
   acid-rinsing briefly with
   water.
4. Counterstain with methylene
   blue for 30 seconds.
5. Rinse briefly with water to
   remove excess methylene
   blue.
6. Blot dry with bibulous paper.
   Examine directly under oil
   immersion.

Question 6

Acid fast stain secondary stain

Answer 6

METHYLENE BLUE/
MALACHITE GREEN

Question 7

Staining method for bacteria that possess ________ in their
cell walls (i.e. Mycobacterium tuberculosis and other species,
and Nocardia)

Answer 7

mycolic acid

Question 8

is used as a decolorizer, and methylene
blue is the counterstain

Answer 8

Acidified alcoho

Question 9

Bacteria that are not acid-fast are color what

Answer 9

blue

Question 10

ZIEHL-NEELSEN mordant

Answer 10

STEAM

Question 11

used to stain bacteria that have a high lipid and wax content in their cell walls and do not stain well
with traditional bacterial stains.

Answer 11

ACID FAST STAINING

Question 12

Acid-fast bacteria retain the primary
stain and are color what

Answer 12

red

Question 13

kinyoun METHYLENE BLUE/
MALACHITE GREEN NAF

Answer 13

blue