Unknown ID - Molecular Methods - Deck (Practical)

Question 1

What are the 3 steps of PCR?

Answer 1

1) Denaturation
2) Annealing/Priming
3) Extension

Question 2

What is the purpose of the denaturation step?

Answer 2

To separate the dsDNA strands

Question 3

What is the purpose of the annealing step?

Answer 3

To bind the primers to the target regions of the ssDNA strands

Question 4

What is the purpose of the extension step?

Answer 4

To extend primers via polymerase and form new dsDNA strands