WBC development & morphology

Question 1

3 morphological variations in neutrophil & their indication

Answer 1

1. Dohle bodies: grey-blue patches in cytoplasm = RNA => indicate immature neutrophil- urgency needed in inflammation
2. Toxic granulation: small basophilic (purple) granules (smaller than basophil granules) - indicate inflammation
3. Band neutrophils: Hyposegmented nuclei (no constrictions) => nuclear immaturity (left-shift)

Question 2

Indication of hypersegmentation in neutrophil morphology

Answer 2

5+ neutrophil w/ 5 lobes OR

automatically when 1+ w/ 6 lobes

Question 3

A morphological variations in lymphocyte & indication of what condition

Answer 3

reactive lymphocyte: kinda looks like monocytes bc irregular outline; decreased N:C ratio
-> indicate mononucleolsis

Question 4

Describe the stages of development of myelopoiesis leading to mature granulocytes in the peripheral blood.

Answer 4

1. Myeloblast
2. Promyelocyte: 1º granules
3. N/B/E myelocyte: 2º granules (dark staining)
4. N/B/E metamyelocyte: nucleus round
5. N/B/E band: nuclei elongated ‘sausage’
6. PMN / basophil / eosinophil: constriction of nuclei