22 - MPC Flashcards

(57 cards)

1
Q
A
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2
Q

What direct method uses Neubauer chamber

A

Reese-Ecker/Tocantins

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3
Q

What prevents platelet clumping in Reese-Ecker diluting fluid

A

sodium+citrate

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4
Q

What stains platelets in Reese-Ecker diluting fluid

A

brilliant+cresyl+blue

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5
Q

What is preservative in Reese-Ecker diluting fluid

A

40percent+formalin

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6
Q

What else is in Reese-Ecker diluting fluid besides citrate stain formalin

A

distilled+water+bicarbonate+sucrose

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7
Q

What is dilution factor for Reese-Ecker

A

1:200

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8
Q

What is modified Reese-Ecker using crystal violet dye

A

Leake+and+Guy

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9
Q

What components in Leake and Guy diluting fluid

A

sodium+citrate+40percent+formalin+crystal+violet+distilled+water

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10
Q

What direct method uses 1percent ammonium oxalate phase contrast microscope 1:100 dilution best direct method

A

Brecher-Cronkite

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11
Q

What system prefilled blood dilution vials preserve cells lyse others premeasured diluent chamber specified blood capillary pipette shield plastic reservoir

A

Unopette

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12
Q

What diluent modified ammonium oxalate Sorensen phosphate buffer thimerosal antibacterial 1percent ammonium oxalate lyse red blood cells platelets pseudopods dilution 1:100

A

Unopette

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13
Q

What Unopette reservoir contains 1.98 mL diluent ammonium oxalate Sorensen phosphate buffer thimerosal purified water

A

Unopette+reservoir

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14
Q

What is Unopette reagent stability stored below 30°C protected sunlight

A

stable

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15
Q

What is Unopette capillary pipette capacity

A

20+microliters

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16
Q

What is platelet count formula initial times 1000

A

platelet+count+x+1000

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17
Q

What specimen free flowing capillary or mixed anticoagulated venous EDTA choice stable 3 hours room temperature

A

platelet+count+specimen

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18
Q

Other direct methods for platelet count

A

Kristenson+Van+Goidsenhoven+Lempert+Masure+Aggeler+Howard+Lisa

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19
Q

What indirect method smear analysis stain Giemsa Wrights ratio 1:3

A

Fonio

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20
Q

What Fonio diluting fluid dry method

A

14percent+magnesium+sulfate

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21
Q

What indirect method wet method

A

Dameshek

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22
Q

What Olef method uses besides Rees Ecker fluid stain brilliant cresyl blue sodium citrate prevent clumping ratio 1:5

A

Olef

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23
Q

What is modified Dameshek uses siliconized medicine dropper dilute blood

A

modified+Dameshek

24
Q

What diluting fluid for modified Dameshek stain Giemsa Wrights same principle Fonio ratio 1:5 best indirect

A

14percent+magnesium+sulfate

25
Manual platelet count make 1:100 dilution 20 microliters blood 1980 microliters 1percent ammonium oxalate small test tube mix charge chamber
procedure
26
What special chamber for phase microscopy platelet counts
thin+flat-bottomed+counting+chamber
27
Place charged hemacytometer moist chamber allow platelets settle
15+minutes
28
Platelets counted using what objective lens total magnification
40x+400x
29
Platelet diameter appearance round oval light purple sheen phase contrast distinguish dirt debris ghost red blood cells
2+to+4+micrometers
30
Count platelets in 25 small squares center square grid area 1 mm² both sides hemacytometer difference less than 10percent
count
31
Accuracy manual platelet count verified by platelet estimate Wright stained peripheral blood
verify
32
Reference value platelet count
150-400+x+10^9/L+or+150-450+x+10^9/L
33
Dilution factor manual platelet count
1:100
34
Squares counted 25/25 or 10 tertiary center square 1mm² area
count+25+small+squares+center
35
Ideal microscope for platelet count
phase+contrast
36
Formula platelet count dilution factor area depth
platelet+count+x+dilution+factor+area+counted+x+0.1+cm
37
1 platelet per how many red blood cells normal smear
10-40
38
3-10 platelets per 100 red blood cells or 1 oil immersion field
platelets+per+100+RBCs
39
5-20 platelets per 200 red blood cells
platelets+per+200+RBCs
40
Normal blood smear platelets per field
8+to+20
41
Platelet estimate factor counted 10 oil immersion fields times
x+20000
42
Less than 100000 per microliter bleeding possible with trauma
30000-50000+per+microliter
43
Less than 30000 per microliter spontaneous bleeding possible
less+than+30000+per+microliter
44
Less than 5000 per microliter severe spontaneous bleeding
less+than+5000+per+microliter
45
Normal platelet count prolonged bleeding time indicates
qualitative+platelet+abnormality+primary+vascular+abnormality+von+Willebrand+disease
46
Low platelet count normal bleeding time indicates
autoimmune+thrombocytopenia
47
Low platelet count very prolonged bleeding time indicates
quantitative+and+qualitative+platelet+deficiency
48
Platelet estimate standard formula average platelets 10 oil immersion fields times
x+20000
49
Platelet estimate anemia erythrocytosis average platelets per field times total red blood cell count
200+RBCs+per+field
50
Report platelet marked decreased 0-49000 per microliter
marked+decreased
51
Report platelet moderate decreased 50000-99000 per microliter
moderate+decreased
52
Report platelet slight decreased 100000-149000 per microliter
slight+decreased
53
Report platelet low normal 150000-199000 per microliter
low+normal
54
Report platelet normal 200000-400000 per microliter
normal
55
Report platelet slight increased 401000-599000 per microliter
slight+increased
56
Report platelet moderate increased 600000-800000 per microliter
moderate+increased
57
Report platelet marked increased above 800000 per microliter
marked+increased