Process of making proteins
Protein Synthesis
Importance of Proteins in Human Body
9 Essential Amino Acids
Histidine
Isoleucine
Lysine
Leucine
Methionine
Phenylalanine
Threonine
Tryptophan
Valine
2 Phases of Protein Synthesis
Transcription and Translation
2 Phases of Protein Synthesis
process by which the information in a strand of DNA is copied into a new molecules of the messager RNA (mRNA)
Transcription
2 Phases of Protein Synthesis
process of translating the sequence of an mRNA molecule to a sequence of amino acids during protein synthesis
Translation
Where and When does Transcription and Translation procesd Go
Where: Cytoplasm
When: Synthesis
the point of origin, dictates the binding of RNA Polymerase II to the DNA
promoter
Steps in Transcription Process
RNA polymerase enzyme binds to and opens the DNA molecule will be transcribed
Initiation
Steps in Transcription Process
As the DNA molecule opens, the RNA polymerase slides along the the DNA strand and links free RNA nucleotides that pair with the nitrogenous bases of the complementary DNA strand
Elongation
Steps in Transcription Process
When the base pairing process is completed, the RNA molecule breaks away as the DNA strands rejoin. The RanA leaves the nucleus and goes to the cytoplasm
Termination
bring the information from the DNA in the nucleus to the cytoplasm
mRNA (messenger RNA)
holds tightly to the mRNA and uses its information to assemble amino acids
rRNA (ribosomal RNA)
a type of RNA that attaches the correct amini acid to the orotein chain that is being synthesized in the ribosome
Transer RNA (tRNA)
each set of 3 nitrogenous bases in mRNA representing an amino acid or start or stop signal
Codon
the complement of the mRNA triplet code in the tRNA
Anticodon
a long chain of amino acids linked by peptide bonds, forming the fundamental structure of proteins
Polypeptide Chaim
Steps in Translation Process
As translation begins, mRNA binds to a ribosome. Then, tRNA molecules, each carrying specific amino acid, approach the ribosome, the tRNA anticodon pairs with the first mRNA (start) codin (AUG) to form the initiation complex. The 2 molecules temporarily join together
Initiation
Steps in Translation Process
A new tRNA molecule carrying an amino acid pair with the second mRNA codon. When the 1st and 2nd amini acids are in place, an enzyme joins them by forming a peptide bond between them
Elongation
Steps in Translation Process
A chain of amino acids is formed until the ribosome reaches a stop codon (UAA,UAG,UGA) on the mRNA strand. The polypeptide chain is released
Termination
Summary of Protein Synthesis
cytoplasm are in the
ribosome
3 Site
P-Site (Peptidyl site)
A-Site (Aminoacyl site)
E-Site (Exit Site)