name 4 potentially infectious agents
SARS-CoV-2, Influenza, yeast, Campylobacter
name the 4 characteristics microorganisms are based on
- pathogenicity
- severity of disease
- risk of spreading into environment
- availability of prophylaxes and treatment
how does the risk classification look like?
name the 2 requirements to call micro-organism a GMO
- artificially modified genetic material
- ability to replicate or transfer genetic material
why are GMOs made?
to study the function of a gene; inactivation, overexpression, mutation, track/trace/measure a gene
name the 2 risks of GMOs
- disturbance of natural balance when introduced into the environment
- transfer of genetically modified material to non-gmo’s
how does the different containment levels look like?
who handles legislation for GMO?
Decree GMO and Regulation GMO
Name the 4 roles of the Biosafety officer (BSO)
- controls and guarantees all activities with potentially infectious organisms
- gives advice and education
- acts in case of GMO incidents and calamities
- acts on behalf of Board of Directors
–> contacted for permit or notification
name the 8 levels of containment
- lab coat
- gloves
- hand washing
- disinfection
- door locked
- autoclave
- biosafety cabinet
- limited entrance
name the 8 pillars of office biosecurity
- awareness
- personnel reliability
- transport security
- information security
- accountability of materials
- response to incidents/emergencies
- management
- physical measures
name the 3 BSL levels
BSL-1; cloning in plasmids, transformation in competent cells
BSL-2; transfection of cells for the production of recombinant virus ‘6+2’-viruses
BSL-3; ‘full 8 segments’
name 7 reasons for gain of function research in viruses
- increase virulence
- overcomes immunity
- develop resistance to drugs
- increase transmission
- change tropism
- increase host susceptibility
- regenerate extinct pathogens
