when should we perform blood collection on bird in exam and why?
Perform as soon as possible
o Limit stress leukogram
o Limit elevation in some enzymes
o Limit elevation in blood glucose and lactates
what can we do to make blood collection easier? How?
Sedation makes it easier
o Midazolam/butorphanol 2/2
mg/kg intranasal
o Reversed with flumazenil 0.05 mg/kg intranasal
> dosages can be species specific
venipuncture sites for avian blood collection
o Right jugular vein (much bigger than left) o (Left jugular vein)
o Ulnar vein
o Medial metatarsal vein
best blood collection site for parrots and small birds of prey, and how many people required
o Right jugular vein preferred
o One person job
best blood collection site for medium to large birds of prey, and how many people required
o Any site
o Two-person job
best blood collection site for anseriformes and galliformes
o Medial metatarsal vein easiest
where not to take blood from for strigiformes? why?
Strigiformes
o Not medial metatarsal (feathered)
where not to take blood from for columbiformes? why?
o Not jugular (no apterium in these species + cervical plexus)
max volume for bird blood collection? what should we be careful of?
o 1% of body weight
o Caution in tiny birds, account for hematoma formation, prior and future blood loss (surgery)
required volume for bloodwork, for CBC, biochem, and vetscan
o CBC: 0.2-0.4 mL
o Biochemistry: 0.2mL plasma (0.4-0.5 blood)
>Don’t use serum, you’ll lose volume
o Vetscan: 0.1mL whole blood - But decreased panel
materials for blood collection in bird
o Smallest needle possible for the volume
harvested
o Needle size: 22-28G
o Syringe size: Insulin – 3cc syringe
technique for blood collection in bird
o Small amount of alcohol
o Stay parallel to the vein, bend the needle, keep the head extended
o Stabilize needle on your thumb or index finger
o Suction steadily with medium speed
- Too slow > clotting
- Too quick > vessel collapse
o Hold for clotting
- Longer pressure for ulnar and medial metatarsal vein
- Bandage
o Remove needle prior to tube transfer
blood collection tubes to use for CBC, bichem
o CBC > EDTA
o Biochemistry > Heparin
- Spin and separate plasma prior to submission
Do not pre-heparinize syringes in what scenarios for bird blood collection
Do not pre-heparinize syringes in small birds or for blood culture
supportive care for birds, main principles
most sick birds require some form of what type of therapy
why is fluid therapy more challenging in birds
o Small size
- Harder to place catheters
- Tiny volume of IV drugs
- Limitations of infusion equipment
o Readily damage IV materials and bandages
o Monitoring is complicated
important osmoregulation considerations in birds for fluid therapy
o Uricotelic
o Post-renal handling of urine
o Salt glands
daily water requirements for birds
o Poorly investigated in pet birds
o 50-100 mg/kg/day
o Higher in neonates
rotues of administration for fluid therapy in birds
PO
SC
IV
IO
PO fluids: sites, pros cons, fluid type
sites: Crop, Proventriculus
pros: Least invasive
cons: GI disease, Neuro disease, Debilitated bird
Fluid type: hypotonic
SC fluids: sites, pros cons, fluid type
sites: Inguinal web,
Interscapular, Axillary area
pros: Non invasive, well tolerated
cons: Mild dehydration, Limited volume
Fluid type: Isotonic, Hypotonic
IV fluids: sites, pros cons, fluid type
sites: Ulnar vein, Medial metatarsal, Jugular vein
pros: IV access for drugs, Rapid dissemination, More precise dosing
cons: Low tolerance, Bleeding if damage
fluid type: Isotonic, Hypotonic ,Hypertonic, Colloid, Blood
IO fluids: sites, pros cons, fluid type
sites: Ulna, Tibiotarsus
pros: No bleeding,
Ideal when veins are small
IV access for drugs Rapid dissemination More precise dosing
cons: Low tolerance, Painful
Potential for osteomyelitis Fluid extravasation
fluid types: Isotonic Hypotonic (Hypertonic) (Colloid) Blood