3RD YR > CC Part 2 > Flashcards

CC Part 2 Flashcards

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1
Q

enzyme with a molecular weight of approximately 82,000 that is generally associated with ATP regeneration in contractile or transport systems

A

Creatine Kinase

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2
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

rhabdomyolysis

A

CK-MM

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3
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

MI

A

CK-MM, MB

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4
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

Duchenne type of muscular dystrophy

A

CK-MB

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5
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

CO Poisoning

A

CK-MB, BB

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6
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

Reye’s syndrome

A

CK-MB, BB

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7
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

CNS shock

A

CK-BB

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8
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

angina

A

CK-MB

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9
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

hypothyroidism

A

CK-MM

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10
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

RMSF

A

CK-MB

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11
Q

identify which isoenzyme is associated with the following condition:

Choices: CK-MM, CK-MB, CK-BB

placental/urine trauma

A

CK-BB

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12
Q

two methods for measuring CK activity

A
  1. Tanzer-Gilvarg
  2. Oliver-Rosalki
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13
Q

cause of false decrease in CK

A

exposure to light
bedridden px

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14
Q

catalyzes the interconversion of lactic and pyruvic acids

A

LD

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15
Q

two methods of measuring LD

A
  1. Wacker method
  2. Wroblueski LaDue
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16
Q

cause of false decrease in LD

A

storing @ 4C (shd be room temp = 25C)

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17
Q

two methods of measuring AST & ALT

A
  1. Karmen Method
  2. Reitman-Frankel Method
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18
Q

optimal pH of ALP

A

9-10

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19
Q

tissue sources of ALP from most anodic to most cathodic

A
  1. Liver
  2. Bone
  3. Placenta
  4. Intestine
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20
Q

cause of false decrease in ALP

A

hypophosphatasia

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21
Q

two isoenzymes of ALP that are heat stable at 65C for 30 mins

A

Regan ALP
Nagao ALP

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22
Q

Methods for ALP & ACP determination

A

Bodansky
King-Armstrong or Cutman-Gutman
Bessey-Lowry-Brock
Hudson
Babson and Reed
Roy
Bowers-McComb
Sinowara-Jones-Reinhart

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23
Q

ACP is particularly significant in the?

A

Prostate (Prostatic ACP)

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24
Q

detects and quantifies antigens or antibodies using enzyme-labeled reactions

A

Enzyme Linked Immunoassay (ELISA)

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25
uses radiolabeled antigens or antibodies for highly sensitive measurement
Radioimmunoassay (RIA)
26
detects antigens by forming visible antigen-antibody complexes
Immunoprecipitation
27
measures analytes using enzyme-labeled antibodies in a sandwich format
Immunoenzymetric assay (IEMA)
28
employs radiolabeled antibodies for direct and sensitive antigen detection
Immunoradiometric (IRMA)
29
uses antibody-coated microparticles to enhance enzyme-based detection
Microparticle enzyme immunoassay
30
source of false decrease error in ACP
delay in analysis increased pH
31
GGT activity is found in highest concentration in the?
kidney
32
Method for ACP determination
Szasz method
33
only performed in very large reference laboratories
5'-NT
34
source of false decrease error in 5'-NT
EDTA
35
dextrin contain what linkages?
a-1-6-branching linkages
36
small enzyme, with a molecular weight of 50,000 to 55,000
Amylase
37
major tissue source of serum AMY
acinar cells of pancreas & salivary glands
38
methods for amylase determination
1. Amyloclastic / Iodometric 2. Saccharogenic 3. Chromolytic 4. Coupled enzymatic
39
optimal pH for AMY activity
6.9
40
source of false decrease error in AMY activity
oxalates & citrates
41
enzyme that hydrolyzes the ester linkages of fats to produce alcohols & fatty acids from triglycerides
lipase
42
Lipase is found primarily in the?
pancreas
43
what is the clinically significant lipase isoenzyme
L2
44
method for lipase determination
1. Turbidimetric 2. Cherry-crandall 3. Tietz-Borden 4. Sigma tietx 5. Colorimetric
45
functions to maintain NADPH in reduced form
Glucose-6-Phosphate Dehydrogenase
46
source of false decrease error in G6PD determination
drug induced hemolytic anemia liver dse insecticide poisoning heart failure malnutrition acute infection anesthesia overdose
47
methods for G6PD determination
1. Michel method 2. Manometric method 3. Ellman Technique
48
liver-specific mitochondrial enzyme
Ornithin Carbamoyl Transferase
49
methods for OCT determination
1. Classic Reichard 2. Reichard isotopic-microdiffusion technique 3. Colorimetric method
50
enzyme that catalyzes the hydrolysis of N-terminal residues fro peptides and amides containing free amino groups
Leucine aminopeptidase
51
determine the class of enzyme G6PD
Oxidoreductase
51
method for LAP determination
1. Goldbarg Fluorometric method 2. Rutenberg Colorimetric method
52
determine the class of enzyme LD
oxidoreductase
53
determine the class of enzyme AST
transferase
54
determine the class of enzyme ALT
transferase
55
determine the class of enzyme OCT
transferase
56
determine the class of enzyme CK
transferase
57
determine the class of enzyme ACP
hydrolase
58
determine the class of enzyme 5'-NT
hydrolase
59
determine the class of enzyme amylase
hydrolase
60
determine the class of enzyme LAP
hydrolase
61
determine the class of enzyme ALP
hydrolase
62
determine the class of enzyme aldolase
lyase
63
determine the class of enzyme TPI
isomerase
64
determine the class of enzyme synthase
ligase
65
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