Routinely performed on CSF specimens
Leukocyte/WBC Count
Adult normal values
0-5 cells/uL
Adult predominant cells
Lymphocytes
Neonates normal values
0-30cells/uL
Neonate’s predominant cells
Monocytes
Methods
Manual
Automated
Manual
Neubauer Counting Chambers
Automated
Automated Cell Counters
QC
Results of manual count should agree with the automated cell count by ±25%
Counting of Cells
4 large corner squares
Large center square
Formula of CSF Cell Counting
Total Cell Count = Cell counted x Dilution / Area x Depth