Needed for the correction of WBC Count and CSF Protein results
RBC Count
RBC is significant in
Traumatic tap
May be counted undiluted
Clear
To dilute possible increase of WBC
NSS
If sample is turbid/milky what should be used
Calibrated, automatic pipettes
Cells may be counted in
4 corner square
Center square
Lyse RBC prior to WBC count [diluent]
3% Glacial Acetic Acid
Methylene blue
RBC counts should be performed
ASAP
what will happen with an hour of delay
RBC will disintegrate within 1hr
what will happen in a 2hrs of delay
Decrease by 40% in WBC
Increase cells in CSF
Pleocytosis