Cell Nutrition Study Guide

Question 1

1. Describe antibody driven cell separation

Answer 1

• Use of specificity of antibodies
• Culture dishes coated with specific antibody
• Cell mixture is added to the dish and cell expressing antigen matching antibodies will attach to the dish while other cells are easily washed away
• Surface needs to have a low binding capacity for unwanted cells

Question 2

2. What are growth factors?

Answer 2

– Molecules in this component group exert their action in minute quantities by binding to specific receptors including signaling pathways that change cell behavior eg: differentiation, migration, secretion, uptake or growth.

Question 3

3. __________________ is the main carrier of nutrients, water, and oxygen to all sites in the body, where the nutrients will diffuse through the capillaries to reach the cells that need them.

Answer 3

blood

Question 4

4. Define anchorage dependent cells. Give an example of the same

Answer 4

cells that need to adhere to a substrate before they start to proliferate

ex fiberblast, skeletal myocytes, chrondrocytes

Question 5

5. Name the three basic types of cell culture media.

Answer 5

simple, complex, and chemically defined media

Question 6

6. Name the three stages of cell culture.

Answer 6

1. expansion of cells
2. initiation of differentiation
3. maintenance of differentiation

Question 7

8. What are suspension cultures

Answer 7

these are cultures that are benifical to certain cell types that do not need to adhere to surface to proliferate and differentiate.

these cells can grow in 3D environments in the this semi solid supsensions.

This culture medium still provides cells with nutrition growth factors

Question 8

9. What is a primary culture?

Answer 8

Cells obtained from animal / human tissue

Question 9

10. T/F During differentiation cells slow down or stop their cell division and mature into a functional phenotype.

Answer 9

True

Question 10

11. What are the two modes of mass transport?

Answer 10

convection and diffusion

Question 11

12. Which type of cells doesn’t need to adhere to a substrate?
    a. Blood cells
    b. Fibroblast
    c. Chondrocytes
    d. Skeletal myocytes

Answer 11

A. blood cells

Question 12

13. Within cell medium components what are the two common sources of protein

Answer 12

serum or (purified) albumin

Question 13

7. Name 2 cell media components

Answer 13

glucose, pyruvate, lactate