cryobiology

Question 1

What is vitrification?

Answer 1

defined as glass like solidification of supporting solutions and living cells that completely avoids ice crystals formation during cooling and warming

Question 2

Why are cryopreservation procedures done?

Answer 2

this is done to…

1. reduce risk of microbial contamination
2. reduce risk of cross contamination with other cell lines

Question 3

Define Cryobiology.

Answer 3

is the science of living organisms, organs biological tissue or biological cells at low temperatures

Question 4

What is the difference between controlled cooling and uncontrolled cooling

Answer 4

controlled cooling=(1C/min), requires controlled rate freezer, which is expensive

uncontrolled cooling-freezer, followed by -80C overnight before transferring to liquid nitrogen for storage

Question 5

What is the purpose of cryoprotectants

Answer 5

to minimize/ prevent cells/tissue or organ from being damage during exposure to low temperature.

forms hydrogen bond to prevent water-water hydrogen bonding that results in ice formation

Question 6

Define aseptic techniques for cell and tissue culture

Answer 6

this is the execution procedures without the introduction of contaminating microorganisms

Question 7

State four ways that a cell culture system can get contaminated

Answer 7

• poor handling
• from contaminated media, reagents, and equipment
• from microorganisms present in incubators, refrigerators, and laminar flow hoods
• from skin of the worker and in cultures from other laboratories

Question 8

Give two methods that you could implement to avoid contamination in cell culture systems

Answer 8

applying good aseptic technique

use antibiotics

keep good house keeping