Chapter 10 Homework

Question 1

Which of the following are steps in sequencing a genome?

Answer 1

fragment the genome, clone the DNA fragments, sequence the DNA fragments

Question 2

Recognition sites for restriction enzymes are

Answer 2

• often palindromes
• may be 4, 6, or 8 bases long

Question 3

Which type of restriction enzyme would produce the smallest fragments

Answer 3

an enzyme that has a recognition site that is 4 bases long

Question 4

Small molecules that serve as carriers for DNA are called

Answer 4

vectors

Question 5

_____ is used to seal the phosphodiester backbones between vector and insert

Answer 5

ligase