Chapter 10 - HPLC method development

Question 1

General 5 step strategy for method development

Answer 1

1. Define method and separation goals
2. Gather sample and analyte information
3. Initial method development (scouting run to get first chromatogram)
4. Method fine tuning and optimization
5. Method prequalification

Question 2

3 types of methods

Answer 2

1. quantitative
2. qualitative
3. preparative

Question 3

A quantitative method generates information on

Answer 3

the concentration or amount of analyte in the sample.

Question 4

Standard internal column diameter for most labs

Answer 4

4.6 mm

Question 5

Does flow rate affect analyte retention or selectivity?

Answer 5

No

Question 6

Increasing column temperature ______ retention and solvent viscosity.

Answer 6

reduces

Question 7

Ideally, the peak height of the main component should be _______ absorbance units.

Answer 7

1-1.5