HPLC
high-performance liquid chromatography
UHPLC
ultra-high-pressure liquid chromatography
tR
retention time
tM
void time
h
peak height
wb
peak width measured at the base
w½
peak width measured at the peak’s half height
N
number of theoretical plates (efficiency)
VR
retention volume
VM
void volume
F
flow rate
Vc
volume of empty column
V0
intraparticular volume
Ve
interstitial volume
k
retention factor
tR’
the time the solute resides in the stationary phase
K
partition coefficient
Vs
volume of the stationary phase
Vm
volume of the mobile phase
𝛼
separation factor
HETP
height equivalent to a theoretical plate
H
plate height
dp
diameter of the particles used as the packing material
Rs
resolution