What are the two major periods of the cell cycle?
Interphase, Mitosis
__________ is the part of the cell cycle that occurs when the cell is not in mitosis.
Interphase
This phase is divided into three parts. __________ is also called gap and is when growth and other metabolic activities occur. __________ is also called synthesis and is when DNA replication occurs. The last portion is called __________ and is when enzymes and other proteins necessary for cell division are produced.
G1,S,G2
DNA replication is _______________ which means that each new strand of DNA is half old and half new DNA
Semiconservative
There are many enzymes that are involved in DNA replication. Which enzyme completes each of the following steps:
a. Forms covalent bonds between DNA segments? __________
b. Adds new DNA nucleotides? __________
c. Builds short complementary pieces of RNA; attracts DNA polymerase to DNA strand? __________
d. Unwinds and unzips DNA double helix? __________
Ligase, DNA Polymerase, Primase, Helicase
How many cells are produced by mitosis?
2 daughter cells
Name the phases of mitosis in the correct order. Be able to label the phases in the diagram below and recognize the parts of the diagram (cell membrane, nucleus, nucleolus, nuclear envelope, chromatin, chromosomes, centrosomes, spindle fibers, cleavage furrow, chromatids)
PMAT Prophase, Metaphase, Anaphase and Telephase
Name the process where the cytoplasm divides.
Cytokinesis
During what stage of mitosis does this take place?
Begins during late anaphase and ends with completion of telophase
Parts of Microscope
Eyepieces, objectives, stage, aperture iris diaphragm ring, condenser, base, light intensity knob, light switch, Mechanical stage controls, fine focus knob, course focus knobs, frame, specimen holder, revolving nosepiece
What is the total magnification if the microscope is set on medium (10X) power?
100x
What happens to the field of view as the magnification increases?
What happens to the working distance as the magnification increases?
Decreases
Decreases
What term is used to describe all of a cell’s genetic material
Genome
_____are coding regions of a gene and __________ are non-coding regions of a gene
Exons, Introns
What is “the central dogma”?
Transcription – cell copies DNA to RNA In nucleus
Translation – RNA manufactures proteins In cytoplasm
DNA-RNA-Protien
Differences in DNA and RNA
Type of sugar
Nitrogenous Bases
Number of Strands
deoxyribose Adenine, Guanine, Thymine, Cytosine 2 ribose, Adenine, guanine, Cytosine Uracil 1
mRNA
MessengerRNA: Carries info from DNA and uses it to help make protein; 3 RNA bases are a codon
tRNA
TransportDNA: Carries specific amino acid to ribosome; allows for amino acid to line up with correct mRNA
sequence
rRNA
Combines with proteins to form a ribosome
Name the three stages of transcription.
Initiation, Elongation, Termination
Where does translation occur?
Cytoplasm
Where does transcription occur?
Inside the nucleus
Programmed cell death is called __________. It is associated with the group of enzymes known as __________.
Apoptosis, Caspases
_________ respiration requires oxygen whereas ________ respiration does not
Aerobic, Anerobic