What are the factors that affect reaction rate of enzyme catalyzed reactions?
Km
The substrate concentration at which vi is half the maximal velocity (vmax/2) attainable at a particular concentration of the enzyme.
Vmax
Maximal Velocity
kcat
Catalytic Constant
kcat/Km
The specificity constant, it tells you “catalytic efficiency” which means how fast in m/s the enzyme reacts with the substrate once it encounters the substrate.
Michaelis-Menten Equation
vi= (Vmax [S]) / (Km + [S])